A collagen-binding EGFR antibody fragment targeting tumors with a collagen-rich extracellular matrix

CORC > 苏州纳米技术与纳米仿生研究所 > 中国科学院苏州纳米技术与纳米仿生研究所 > 纳米生物医学与安全研究部 > 戴建武团队

	A collagen-binding EGFR antibody fragment targeting tumors with a collagen-rich extracellular matrix
	Liang, H(梁辉); Li, XR(李晓然); Wang, B; Chen, B; Zhao, YN; Sun, J(孙杰); Zhuang, Y(庄燕); Shi, JJ(施家佳); Shen, H(沈贺); Zhang, ZJ(张智军)
刊名	SCIENTIFIC REPORTS
	2016
卷号	6
通讯作者	Dai, JW(戴建武)
英文摘要	Many tumors over-express collagen, which constitutes the physical scaffold of tumor microenvironment. Collagen has been considered to be a target for cancer therapy. The collagen-binding domain (CBD) is a short peptide, which could bind to collagen and achieve the sustained release of CBD-fused proteins in collagen scaffold. Here, a collagen-binding EGFR antibody fragment was designed and expressed for targeting the collagen-rich extracellular matrix in tumors. The antibody fragment (Fab) of cetuximab was fused with CBD (CBD-Fab) and expressed in Pichia pastoris. CBD-Fab maintained antigen binding and anti-tumor activity of cetuximab and obtained a collagen-binding ability in vitro. The results also showed CBD-Fab was mainly enriched in tumors and had longer retention time in tumors in A431 s.c. xenografts. Furthermore, CBD-Fab showed a similar therapeutic efficacy as cetuximab in A431 xenografts. Although CBD-Fab hasn't showed better therapeutic effects than cetuximab, its smaller molecular and special target may be applicable as antibody-drug conjugates (ADC) or immunotoxins.
关键词[WOS]	GROWTH-FACTOR RECEPTOR ; PICHIA-PASTORIS ; MONOCLONAL-ANTIBODIES ; CANCER-THERAPY ; IN-VIVO ; MICROENVIRONMENTAL REGULATION ; PROGRESSION ; EFFICACY ; INVASION ; MECHANISMS
收录类别	SCI
语种	英语
WOS记录号	WOS:000370234500001
内容类型	期刊论文
源URL	[http://ir.sinano.ac.cn/handle/332007/4784]
专题	苏州纳米技术与纳米仿生研究所_纳米生物医学与安全研究部_戴建武团队
推荐引用方式 GB/T 7714	Liang, H,Li, XR,Wang, B,et al. A collagen-binding EGFR antibody fragment targeting tumors with a collagen-rich extracellular matrix[J]. SCIENTIFIC REPORTS,2016,6.
APA	Liang, H.,Li, XR.,Wang, B.,Chen, B.,Zhao, YN.,...&Dai, JW.(2016).A collagen-binding EGFR antibody fragment targeting tumors with a collagen-rich extracellular matrix.SCIENTIFIC REPORTS,6.
MLA	Liang, H,et al."A collagen-binding EGFR antibody fragment targeting tumors with a collagen-rich extracellular matrix".SCIENTIFIC REPORTS 6(2016).