Biochemical and kinetic evaluation of the enzymatic toxins from two stinging scyphozoans Nemopilema nomurai and Cyanea nozakii

	Biochemical and kinetic evaluation of the enzymatic toxins from two stinging scyphozoans Nemopilema nomurai and Cyanea nozakii
	Yue, Yang1,2 ; Yu, Huahua1 ; Li, Rongfeng 1; Xing, Ronge1 ; Liu, Song 1; Li, Kecheng 1; Wang, Xueqin 1; Chen, Xiaolin 1; Li, Pengcheng1
刊名	TOXICON
	2017
卷号	125 页码:1-12
关键词	Jellyfish venom Enzymatic toxins Enzyme kinetics Metalloproteinase Nemopilema nomurai Cyanea nozakii
英文摘要	Jellyfish envenomations are emerging as an important public health concern occurred worldwide. In China, the situation is getting worse with numerous people stung by jellyfish Nemopilema nomurai (N. nomurai) and Cyanea nozakii (C. nozakii) in the summer. However, the proteinaceous mixtures in nematocysts responsible for the symptoms of jellyfish stings were scarcely characterized and understood in view of enzymatic constituents and toxicity. In the present study, enzymatic properties of jellyfish N. nomurai and C. nozakii nematocyst venom were analyzed biochemically and kinetically. The current data revealed that N. nomurai and C. nozakii nematocyst venom exhibited various enzymatic activities, of which metalloproteinases activity and PLA(2)s-like activity were predominant. Moreover, the catalytic activities of metalloproteinases and PLA(2)s-like were dependent on different physiochemical conditions such as temperature, pH and divalent ions. Kinetic profiling revealed their catalytic behaviors fitted the Michaelis-Menten equation under specific conditions. Findings suggested jellyfish nematocyst venom possessed diverse enzymatic constituents, which may underlie the extensively characterized bioactivities of jellyfish venom and human envenomations. Hence, our study will contribute to understanding the enzymatic constituents and toxicity of jellyfish nematocyst venom and may afford potential therapeutic targets for developing drugs for jellyfish stings. (C) 2016 Elsevier Ltd. All rights reserved.
收录类别	SCI
语种	英语
WOS记录号	WOS:000392895800001
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.qdio.ac.cn/handle/337002/136307]
专题	海洋研究所_海洋生物技术研发中心海洋研究所_实验海洋生物学重点实验室海洋研究所_海洋环境工程技术研究发展中心
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, 19 Yuquan Rd, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Yue, Yang,Yu, Huahua,Li, Rongfeng,et al. Biochemical and kinetic evaluation of the enzymatic toxins from two stinging scyphozoans Nemopilema nomurai and Cyanea nozakii[J]. TOXICON,2017,125:1-12.
APA	Yue, Yang.,Yu, Huahua.,Li, Rongfeng.,Xing, Ronge.,Liu, Song.,...&Li, Pengcheng.(2017).Biochemical and kinetic evaluation of the enzymatic toxins from two stinging scyphozoans Nemopilema nomurai and Cyanea nozakii.TOXICON,125,1-12.
MLA	Yue, Yang,et al."Biochemical and kinetic evaluation of the enzymatic toxins from two stinging scyphozoans Nemopilema nomurai and Cyanea nozakii".TOXICON 125(2017):1-12.