Variation in the ovine PRKAG3 gene (SCI)

CORC > 寒区旱区环境与工程研究所 > 中科院寒区旱区环境与工程研究所（未分类） > 期刊论文

	Variation in the ovine PRKAG3 gene (SCI)
	Yang G.; Zhou H. T.; Wang R. Y.; Hickford J.
刊名	Gene
	2015
卷号	567 期号:2 页码:251-254
英文摘要	The 5'AMP-activated protein kinase (AMPK) is a heterotrimeric enzyme that controls cellular energy homeostasis in response to environmental or nutritional stress. The PRKAG3 gene (PRICAG3) encodes the gamma 3 subunit of the AMPK. Variation in this gene has been found to be associated with meat quality traits in pigs. In this study, we used polymerase chain reaction-single stranded conformational polymorphism (PCR-SSCP) to investigate variation in exon 3 and exons 4-6 of ovine PRKAG3. In 160 New Zealand Suffolk sheep, two variant sequences (named a and b) were identified in the exon 3 region of the gene and three variant sequences (named A, B and C) were identified in the exon 4-6 region of the gene, respectively. A total of three nucleotide substitutions were revealed and these were located in intron 4, exon 4 and intron 5, respectively. The nucleotide substitution identified in the exon 4 (g.2656 C>T) could nominally lead to an amino acid substitution of tryptophan to arginine at position 230 (R230W) in ovine PRKAG3. In comparison with the PRKAG3 amino add sequences in other species, this R230W substitution appeared to occur only in sheep. This is the first report of genetic variation in ovine PRICAG3, and the variation found in this study could be functionally important for AMPK activity, which in turn may affect meat quality traits in sheep. (C) 2015 Elsevier B.V. All rights reserved.
收录类别	SCI
语种	英语
内容类型	期刊论文
源URL	[http://ir.casnw.net/handle/362004/27423]
专题	寒区旱区环境与工程研究所_中科院寒区旱区环境与工程研究所（未分类）_期刊论文
推荐引用方式 GB/T 7714	Yang G.,Zhou H. T.,Wang R. Y.,et al. Variation in the ovine PRKAG3 gene (SCI)[J]. Gene,2015,567(2):251-254.
APA	Yang G.,Zhou H. T.,Wang R. Y.,&Hickford J..(2015).Variation in the ovine PRKAG3 gene (SCI).Gene,567(2),251-254.
MLA	Yang G.,et al."Variation in the ovine PRKAG3 gene (SCI)".Gene 567.2(2015):251-254.