miR-9-5p miR-675-5p and miR-138-5p Damages the Strontium and LRP5-Mediated Skeletal Cell Proliferation Differentiation and Adhesion

	miR-9-5p miR-675-5p and miR-138-5p Damages the Strontium and LRP5-Mediated Skeletal Cell Proliferation Differentiation and Adhesion
	Sun, Tianhao; Leung, Frankie; Lu, William W.
刊名	INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
	2016
英文摘要	This study was designed to evaluate the effects of strontium on the expression levels of microRNAs (miRNAs) and to explore their effects on skeletal cell proliferation, differentiation, adhesion, and apoptosis.The targets of these miRNAs were also studied. Molecular cloning, cell proliferation assay, cell apoptosis assay, quantitative real-time PCR, and luciferase reporter assay were used. Strontium altered theexpression levels of miRNAs in vitro and in vivo. miR-9-5p, miR-675-5p, and miR-138-5p impaired skeletalcell proliferation, cell differentiation and cell adhesion. miR-9-5p and miR-675-5p induced MC3T3-E1 cellapoptosis more specifically than miR-138-5p. miR-9-5p, miR-675-5p, and miR-138-5p targeted glycogen synthase kinase 3 (GSK3), ATPase Aminophospholipid Transporter Class I Type 8A Member 2 (ATP8A2), and Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1), respectively. Low-density lipoprotein receptor-related protein 5 (LRP5) played a positive role in skeletal development. miR-9-5p, miR-675-5p, and miR-138-5p damage strontium and LRP5-mediated skeletal cell proliferation, differentiation, andadhesion, and induce cell apoptosis by targeting GSK3, ATP8A2, and EIF4EBP1, respectively.
收录类别	SCI
原文出处	http://www.mdpi.com/1422-0067/17/2/236/htm
语种	英语
内容类型	期刊论文
源URL	[http://ir.siat.ac.cn:8080/handle/172644/10771]
专题	深圳先进技术研究院_医药所
作者单位	INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
推荐引用方式 GB/T 7714	Sun, Tianhao,Leung, Frankie,Lu, William W.. miR-9-5p miR-675-5p and miR-138-5p Damages the Strontium and LRP5-Mediated Skeletal Cell Proliferation Differentiation and Adhesion[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2016.
APA	Sun, Tianhao,Leung, Frankie,&Lu, William W..(2016).miR-9-5p miR-675-5p and miR-138-5p Damages the Strontium and LRP5-Mediated Skeletal Cell Proliferation Differentiation and Adhesion.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES.
MLA	Sun, Tianhao,et al."miR-9-5p miR-675-5p and miR-138-5p Damages the Strontium and LRP5-Mediated Skeletal Cell Proliferation Differentiation and Adhesion".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2016).