Stromal Interaction Molecule 1 rescues store-operated calcium entry and protects NG115-401L cells against cell death induced by endoplasmic reticulum and mitochondrial oxidative stress

	Stromal Interaction Molecule 1 rescues store-operated calcium entry and protects NG115-401L cells against cell death induced by endoplasmic reticulum and mitochondrial oxidative stress
	Zhang, Changfeng; Thomas, David W.
刊名	NEUROCHEMISTRY INTERNATIONAL
	2016
英文摘要	In this study we sought to investigate the hypothesis that expression of the Stromal Interaction Molecule 1(STIM1) could provide protection against cell death induced by ER and oxidative stress. STIM1 performs an essential role in regulating store operated calcium entry (SOCE) and thereby provides an important route for replenishment of endoplasmic reticulum (ER) Ca2+ stores. We used NG115-401L as a model neuronal cellphenotype with a predicted high susceptibility to ER stress due to SOCE deficiency and the absence of STIM1 expression. We show that STIM1 rescue vigorously re-establishes SOCE responses inducible bysarcoplasmic/endoplasmic reticulum Ca2+-ATPase (SERCA) blockers and Ca2+-linked receptors, producing a useful cell line with a simple STIM1/SOCE on/off switch. Surprisingly, we find that expressing STIM1 in NG115-401L cells appears to not have a significant impact on stored ER Ca2+ levels. Yet, even though we find no evidence for an influence on ER Ca2+ levels, we observed that provision of STIM1 function and rescue of SOCE activity produced a neuronal phenotype with significantly greater resistance to ERstress induced by SERCA blockade. Moreover, we also report that STIM1 expression, despite elevatingmitochondrial reactive oxygen species, endows the NG115-401L neuronal cells with significant resistance to agents that mediate glutathione depletion and subsequent oxidative stress induced apoptosis. Our findings thus suggest that STIM1 warrants further investigation as a potential mediator of neuroprotective pathwaysagainst ER and oxidative stress.
收录类别	SCI
原文出处	http://www.sciencedirect.com/science/article/pii/S019701861630050X
语种	英语
内容类型	期刊论文
源URL	[http://ir.siat.ac.cn:8080/handle/172644/10725]
专题	深圳先进技术研究院_医药所
作者单位	NEUROCHEMISTRY INTERNATIONAL
推荐引用方式 GB/T 7714	Zhang, Changfeng,Thomas, David W.. Stromal Interaction Molecule 1 rescues store-operated calcium entry and protects NG115-401L cells against cell death induced by endoplasmic reticulum and mitochondrial oxidative stress[J]. NEUROCHEMISTRY INTERNATIONAL,2016.
APA	Zhang, Changfeng,&Thomas, David W..(2016).Stromal Interaction Molecule 1 rescues store-operated calcium entry and protects NG115-401L cells against cell death induced by endoplasmic reticulum and mitochondrial oxidative stress.NEUROCHEMISTRY INTERNATIONAL.
MLA	Zhang, Changfeng,et al."Stromal Interaction Molecule 1 rescues store-operated calcium entry and protects NG115-401L cells against cell death induced by endoplasmic reticulum and mitochondrial oxidative stress".NEUROCHEMISTRY INTERNATIONAL (2016).