Synthesis and characterization of low molecular weight polyethyleneimine-terminated Poly(b-amino ester) for highly efficient gene delivery of minicircle DNA | |
Jing Zhao; Lei Yang; Ping Huang; ZhiYong Wang; Yan Tan; Hong Liu; JiaJia Pan; Cheng-Yi He; Zhi-Ying Chen | |
刊名 | Journal of Colloid and Interface Science
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2016 | |
英文摘要 | Gene therapy has held great promise for treating specific acquired and inherited diseases. However, the lack of safe and efficient gene delivery systems remains as the major challenge. Poly(b-amino ester)s (PBAEs) have attracted much attention due to their outstanding properties in biosafety, DNA delivery efficiency and convenience in synthesis. In this paper, we reported the further enhancement of the PBAE functions by increasing its positive charge through conjugating with low molecular weight polyethylenimine (LPEI). The resulted LPEI–PBAE polymer was able to condense minicircle DNA (mcDNA) forming nanoparticles with a diameter of 50–200 nm. Furthermore, as compared to parental PBAE and a commercial transfection reagent very common in laboratory application, the LPEI–PBAE demonstrated significantly higher transfection efficiency with little cytotoxicity. These results suggested LPEI–PBAEs are worthy of further optimization for gene therapy applications. |
收录类别 | SCI |
原文出处 | http://www.pubfacts.com/detail/26520815/Synthesis-and-characterization-of-low-molecular-weight-polyethyleneimine-terminated-Poly-amino-ester |
语种 | 英语 |
内容类型 | 期刊论文 |
源URL | [http://ir.siat.ac.cn:8080/handle/172644/10716] ![]() |
专题 | 深圳先进技术研究院_医药所 |
作者单位 | Journal of Colloid and Interface Science |
推荐引用方式 GB/T 7714 | Jing Zhao,Lei Yang,Ping Huang,et al. Synthesis and characterization of low molecular weight polyethyleneimine-terminated Poly(b-amino ester) for highly efficient gene delivery of minicircle DNA[J]. Journal of Colloid and Interface Science,2016. |
APA | Jing Zhao.,Lei Yang.,Ping Huang.,ZhiYong Wang.,Yan Tan.,...&Zhi-Ying Chen.(2016).Synthesis and characterization of low molecular weight polyethyleneimine-terminated Poly(b-amino ester) for highly efficient gene delivery of minicircle DNA.Journal of Colloid and Interface Science. |
MLA | Jing Zhao,et al."Synthesis and characterization of low molecular weight polyethyleneimine-terminated Poly(b-amino ester) for highly efficient gene delivery of minicircle DNA".Journal of Colloid and Interface Science (2016). |
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