Synthesis and characterization of low molecular weight polyethyleneimine-terminated Poly(b-amino ester) for highly efficient gene delivery of minicircle DNA

	Synthesis and characterization of low molecular weight polyethyleneimine-terminated Poly(b-amino ester) for highly efficient gene delivery of minicircle DNA
	Jing Zhao; Lei Yang; Ping Huang; ZhiYong Wang; Yan Tan; Hong Liu; JiaJia Pan; Cheng-Yi He; Zhi-Ying Chen
刊名	Journal of Colloid and Interface Science
	2016
英文摘要	Gene therapy has held great promise for treating specific acquired and inherited diseases. However, the lack of safe and efficient gene delivery systems remains as the major challenge. Poly(b-amino ester)s (PBAEs) have attracted much attention due to their outstanding properties in biosafety, DNA delivery efficiency and convenience in synthesis. In this paper, we reported the further enhancement of the PBAE functions by increasing its positive charge through conjugating with low molecular weight polyethylenimine (LPEI). The resulted LPEI–PBAE polymer was able to condense minicircle DNA (mcDNA) forming nanoparticles with a diameter of 50–200 nm. Furthermore, as compared to parental PBAE and a commercial transfection reagent very common in laboratory application, the LPEI–PBAE demonstrated significantly higher transfection efficiency with little cytotoxicity. These results suggested LPEI–PBAEs are worthy of further optimization for gene therapy applications.
收录类别	SCI
原文出处	http://www.pubfacts.com/detail/26520815/Synthesis-and-characterization-of-low-molecular-weight-polyethyleneimine-terminated-Poly-amino-ester
语种	英语
内容类型	期刊论文
源URL	[http://ir.siat.ac.cn:8080/handle/172644/10716]
专题	深圳先进技术研究院_医药所
作者单位	Journal of Colloid and Interface Science
推荐引用方式 GB/T 7714	Jing Zhao,Lei Yang,Ping Huang,et al. Synthesis and characterization of low molecular weight polyethyleneimine-terminated Poly(b-amino ester) for highly efficient gene delivery of minicircle DNA[J]. Journal of Colloid and Interface Science,2016.
APA	Jing Zhao.,Lei Yang.,Ping Huang.,ZhiYong Wang.,Yan Tan.,...&Zhi-Ying Chen.(2016).Synthesis and characterization of low molecular weight polyethyleneimine-terminated Poly(b-amino ester) for highly efficient gene delivery of minicircle DNA.Journal of Colloid and Interface Science.
MLA	Jing Zhao,et al."Synthesis and characterization of low molecular weight polyethyleneimine-terminated Poly(b-amino ester) for highly efficient gene delivery of minicircle DNA".Journal of Colloid and Interface Science (2016).