Self-adjuvanted nanovaccine for cancer immunotherapy: Role of lysosomal rupture-induced ROS in MHC class I antigen presentation

	Self-adjuvanted nanovaccine for cancer immunotherapy: Role of lysosomal rupture-induced ROS in MHC class I antigen presentation
	Wang, Ce; Li, Ping; Liu, Lanlan; Pan, Hong; Li, Hongchang; Cai, Lintao; Ma, Yifan
刊名	BIOMATERIALS
	2016
英文摘要	MHC class I (MHC I) antigen presentation of exogenous antigens (so called "cross presentation") is a central mechanism of CD8(+) cytotoxic T lymphocyte (CTL) responses essential for successful vaccine based cancer immunotherapy. The present study constructed amphiphilic pH-sensitive galactosyl dextran-retinal (GDR) nanogels for cancer vaccine delivery, in which dextran was conjugated with all trans retinal (a metabolite of vitamin A) through a pH-sensitive hydrazone bond, followed by galactosylation to acquire dendritic cell (DC)-targeting ability. Our results showed that pH-sensitive GDR nanogel was a self-adjuvanted vaccine carrier that not only promoted DC maturation through activating retinoic acid receptor (RAR) signaling, but also facilitated antigen uptake and cytosolic antigen release in DCs. Furthermore, pH-sensitive GDR nanogel effectively augmented MHC I antigenpresentation and evoked potent anti-cancer immune responses in vivo. More importantly, we first reported that nanoparticle-triggered lysosome rupturecould directly induce ROS production in DCs, which was found to be essential for augmenting proteasome activity and downstream MHC I antigenpresentation. Hence, DC-targeted pH-sensitive GDR nanogels could be a potent delivery system for cancer vaccine development. Triggering lyososomalrupture in DCs with pH-sensitive nanoparticles might be a plausible strategy to elevate intracellular ROS production for promoting antigen crosspresentation, thereby improving cancer vaccine efficacy
收录类别	SCI
原文出处	http://www.sciencedirect.com/science/article/pii/S0142961215009503
语种	英语
内容类型	期刊论文
源URL	[http://ir.siat.ac.cn:8080/handle/172644/10652]
专题	深圳先进技术研究院_医药所
作者单位	BIOMATERIALS
推荐引用方式 GB/T 7714	Wang, Ce,Li, Ping,Liu, Lanlan,et al. Self-adjuvanted nanovaccine for cancer immunotherapy: Role of lysosomal rupture-induced ROS in MHC class I antigen presentation[J]. BIOMATERIALS,2016.
APA	Wang, Ce.,Li, Ping.,Liu, Lanlan.,Pan, Hong.,Li, Hongchang.,...&Ma, Yifan.(2016).Self-adjuvanted nanovaccine for cancer immunotherapy: Role of lysosomal rupture-induced ROS in MHC class I antigen presentation.BIOMATERIALS.
MLA	Wang, Ce,et al."Self-adjuvanted nanovaccine for cancer immunotherapy: Role of lysosomal rupture-induced ROS in MHC class I antigen presentation".BIOMATERIALS (2016).