Neurotoxin-directed synthesis and in vitro evaluation of Au nanoclusters

	Neurotoxin-directed synthesis and in vitro evaluation of Au nanoclusters
	Zhengbo Sun; Wenlu Zhang; Pengfei Zhang; Duyang Gao; Ping Gong; Xue-Feng Yu; Yingliang Wu; Zhijian Cao; Wenxin Li; Lintao Cai
刊名	RSC Advances
	2015
英文摘要	Multifunctional theranostic materials with good biocompatibility are desirable for cancer imaging and therapy. In this paper, a glioma-specific theranostic agent is prepared using Chlorotoxin fusion protein GST-CTX (gCTX) as a template to direct the synthesis of Au nanoclusters (NCs). By trapping the Au NCs in the gCTX, the prepared Au@gCTX NCs show ultrasmall size with hydrodynamic diameter of 2.2 nm and exhibit red-emitting fluorescence with a quantum yield of approximately 6.5%. The investigation from confocal fluorescent microscopy reveals the bright fluorescence and high specificity of the Au@gCTX NCs to label glioma cells through binding to membrane-bound matrix metalloproteinase 2 (MMP-2). Gelatin Zymography, MTT cell viability assay and flow cytometry studies further demonstrate that the Au@gCTX NCs can inhibit the enzymatic activity of MMP-2 and cancer proliferation by elevating intracellular ROS levels, and without harming normal cells. Our results suggest an efficient method for the synthesis of multifunctional theranostic agents for the treatment of cancer.
收录类别	SCI
原文出处	http://pubs.rsc.org/en/content/articlepdf/2015/ra/c5ra03006d?page=search
语种	英语
内容类型	期刊论文
源URL	[http://ir.siat.ac.cn:8080/handle/172644/7344]
专题	深圳先进技术研究院_医药所
作者单位	RSC Advances
推荐引用方式 GB/T 7714	Zhengbo Sun,Wenlu Zhang,Pengfei Zhang,et al. Neurotoxin-directed synthesis and in vitro evaluation of Au nanoclusters[J]. RSC Advances,2015.
APA	Zhengbo Sun.,Wenlu Zhang.,Pengfei Zhang.,Duyang Gao.,Ping Gong.,...&Lintao Cai.(2015).Neurotoxin-directed synthesis and in vitro evaluation of Au nanoclusters.RSC Advances.
MLA	Zhengbo Sun,et al."Neurotoxin-directed synthesis and in vitro evaluation of Au nanoclusters".RSC Advances (2015).