Surrogate target cells expressing surface anti-idiotype antibody for the clinical evaluation of an internalizing CD22-specific antibody

	Surrogate target cells expressing surface anti-idiotype antibody for the clinical evaluation of an internalizing CD22-specific antibody
	Leung Shuion; Gao Kai; Wang Guang Yu; Cheung Benny Kawa; Lee Kwanyeung; Zhao Qi; Cheung WingTai; Wang Jun Zhi
刊名	MABS
	2015
英文摘要	SM03, a chimeric antibody that targets the B-cell restricted antigen CD22, is currently being clinically evaluated for the treatment of lymphomas and other autoimmune diseases in China. SM03 binding to surface CD22 leads to rapid internalization, making the development of an appropriate cell-based bioassay for monitoring changes in SM03 bioactivities during production, purification, storage, and clinical trials difficult. We report herein the development of an anti-idiotype antibody against SM03. Apart from its being used as a surrogate antigen for monitoring SM03 binding affinities, the anti-idiotype antibody was engineered to express as fusion proteins on cell surfaces in a non-internalizing manner, and the engineered cells were used as novel surrogate target cells for SM03. SM03-induced complement-mediated cytotoxicity (CMC) against these surrogate target cells proved to be an effective bioassay for monitoring changes in Fc functions, including those resulting from minor structural modifications borne within the Fc-appended carbohydrates. The approach can be generally applied for antibodies that target rapidly internalizing or non-surface bound antigens. The combined use of the anti-idiotype antibody and the surrogate target cells could help evaluate clinical parameters associated with safety and efficacies, and possibly the mechanisms of action of SM03.
收录类别	SCI
原文出处	http://www.ncbi.nlm.nih.gov/pubmed/25427174
语种	英语
内容类型	期刊论文
源URL	[http://ir.siat.ac.cn:8080/handle/172644/7321]
专题	深圳先进技术研究院_医药所
作者单位	MABS
推荐引用方式 GB/T 7714	Leung Shuion,Gao Kai,Wang Guang Yu,et al. Surrogate target cells expressing surface anti-idiotype antibody for the clinical evaluation of an internalizing CD22-specific antibody[J]. MABS,2015.
APA	Leung Shuion.,Gao Kai.,Wang Guang Yu.,Cheung Benny Kawa.,Lee Kwanyeung.,...&Wang Jun Zhi.(2015).Surrogate target cells expressing surface anti-idiotype antibody for the clinical evaluation of an internalizing CD22-specific antibody.MABS.
MLA	Leung Shuion,et al."Surrogate target cells expressing surface anti-idiotype antibody for the clinical evaluation of an internalizing CD22-specific antibody".MABS (2015).