Homogeneous and Label-Free Detection of MicroRNAs Using Bifunctional Strand Displacement Amplification-Mediated Hyperbranched Rolling Circle Amplification

	Homogeneous and Label-Free Detection of MicroRNAs Using Bifunctional Strand Displacement Amplification-Mediated Hyperbranched Rolling Circle Amplification
	Zhang, Li-rong; Zhu, Guichi; Zhang, Chun-yang
刊名	ANALYTICAL CHEMISTRY
	2014
英文摘要	MicroRNAs (miRNAs) are an emerging class of biomarkers and therapeutic targets for various diseases including cancers. Here, we develop a homogeneousand label-free method for sensitive detection of let-7a miRNA based on bifunctional strand displacement amplification (SDA)-mediated hyperbranched rollingcircle amplification (HRCA). The binding of target miRNA with the linear template initiates the bifunctional SDA reaction, generating two different kinds oftriggers which can hybridize with the linear template to initiate new rounds of SDA reaction for the production of more and more triggers. In the meantime, the released two different kinds of triggers can function as the first and the second primers, respectively, to initiate the HRCA reaction whose products can be simply monitored by a standard fluorometer with SYBR Green I as the fluorescent indicator. The proposed method exhibits high sensitivity with a detectionlimit of as low as 1.8 X 10(-13) M and a large dynamic range of 5 orders of magnitude from 0.1 pM to 10 nM, and it can even discriminate the single-base difference among the miRNA family members. Moreover, this method can be used to analyze the total RNA samples from the human lung tissues and might be further applied for sensitive detection of various proteins, small molecules, and metal ions in combination with specific aptamers.
收录类别	SCI
原文出处	http://pubs.acs.org/doi/abs/10.1021/ac501645x
语种	英语
内容类型	期刊论文
源URL	[http://ir.siat.ac.cn:8080/handle/172644/6136]
专题	深圳先进技术研究院_医药所
作者单位	ANALYTICAL CHEMISTRY
推荐引用方式 GB/T 7714	Zhang, Li-rong,Zhu, Guichi,Zhang, Chun-yang. Homogeneous and Label-Free Detection of MicroRNAs Using Bifunctional Strand Displacement Amplification-Mediated Hyperbranched Rolling Circle Amplification[J]. ANALYTICAL CHEMISTRY,2014.
APA	Zhang, Li-rong,Zhu, Guichi,&Zhang, Chun-yang.(2014).Homogeneous and Label-Free Detection of MicroRNAs Using Bifunctional Strand Displacement Amplification-Mediated Hyperbranched Rolling Circle Amplification.ANALYTICAL CHEMISTRY.
MLA	Zhang, Li-rong,et al."Homogeneous and Label-Free Detection of MicroRNAs Using Bifunctional Strand Displacement Amplification-Mediated Hyperbranched Rolling Circle Amplification".ANALYTICAL CHEMISTRY (2014).