Enhanced gene delivery by chitosan-disulfide-conjugated LMW-PEI for facilitating osteogenic differentiation

	Enhanced gene delivery by chitosan-disulfide-conjugated LMW-PEI for facilitating osteogenic differentiation
	Zhao, Xiaoli; Li, Zhaoyang; Pan, Haobo; Liu, Wenguang; Lv, Minmin; Leung, Frankie; Lu, William W.
刊名	ACTA BIOMATERIALIA
	2013
英文摘要	Chitosan-disulfide-conjugated LMW-PEI (CS-ss-PEI) was designed to combine the biocompatibility of chitosan and the gene delivery ability of polyethylenimine (PEI) using bio-reducible disulfide for bone morphogenetic protein (BMP2) gene delivery in mediating osteogenic differentiation. It was prepared by conjugating low molecular weight PEI (LMW-PEI) to chitosan through oxidization of thiols introduced for the formation of disulfide linkage. The structure, molecular weight and buffer capacity were characterized by Fourier transform infrared (FTIR), light scattering and acid-base titration, respectively. The reduction in molecular weight of CS-ss-PEI by the reducing agent indicated its bio-reducible property. With the increment in the LMW-PEI component, the copolymer showed increased DNA binding ability and formed denser nanocomplexes. CS-ss-PEI exhibited low cytotoxicity in COS-1, HepG2 and 293T cells over the different weight ratios. The transfection efficiency of CS-ss-PEI4 was significantly higher than that of PEI 25k and comparable with Lipofectamine in mediating luciferase expression. Its application for BMP2 gene delivery was confirmed in C2C12 cells by BMP2 expression. For inducing in vitro osteogenic differentiation, CS-ss-PEI4 mediated BMP2 gene delivery showed a stronger effect in MG-63 osteoblast cells and stem cells in terms of alkaline phosphatase activity and mineralization compared with PEI25k and Lipofectamine. This study provides a potential gene delivery system for orthopedic-related disease. (C) 2013 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
收录类别	SCI
原文出处	http://www.sciencedirect.com/science/article/pii/S1742706113000548
语种	英语
内容类型	期刊论文
源URL	[http://ir.siat.ac.cn:8080/handle/172644/5197]
专题	深圳先进技术研究院_医药所
作者单位	ACTA BIOMATERIALIA
推荐引用方式 GB/T 7714	Zhao, Xiaoli,Li, Zhaoyang,Pan, Haobo,et al. Enhanced gene delivery by chitosan-disulfide-conjugated LMW-PEI for facilitating osteogenic differentiation[J]. ACTA BIOMATERIALIA,2013.
APA	Zhao, Xiaoli.,Li, Zhaoyang.,Pan, Haobo.,Liu, Wenguang.,Lv, Minmin.,...&Lu, William W..(2013).Enhanced gene delivery by chitosan-disulfide-conjugated LMW-PEI for facilitating osteogenic differentiation.ACTA BIOMATERIALIA.
MLA	Zhao, Xiaoli,et al."Enhanced gene delivery by chitosan-disulfide-conjugated LMW-PEI for facilitating osteogenic differentiation".ACTA BIOMATERIALIA (2013).