Isothermally sensitive detection of serum circulating miRNAs for lung cancer diagnosis | |
Li, Ying; Liang, Li; Zhang, Chun-yang | |
刊名 | ANALYTICAL CHEMISTRY
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2013 | |
英文摘要 | Tumor-derived miRNAs in serum are emerging as the new noninvasive biomarkers for the diagnosis of human cancers, especially at their early stage. An ideal method with high sensitivity, excellent selectivity, a simple procedure, and small amounts of starting materials is imperative for the detection of cliniccirculating miRNAs. Here, we develop a new method for isothermally sensitive detection of serum miRNAs using hairpin probe-based rolling circle amplification (HP-RCA). This method exhibits ultrahigh sensitivity toward lung cancer-related miR-486-5p with a detection limit of as low as 10 fM and a large dynamic rangeof 6 orders of magnitude, and it can even discriminate miR-486-5p from both miRNAs with high sequence homology and its precursors (pre-miRNAs). More importantly, this method can directly and accurately distinguish the expression of serum miR-486-5p among six nonsmall-cell lung carcinoma (NSCLC) patients and six healthy persons, holding a great potential for further applications in the clinical diagnosis of lung cancers. |
收录类别 | SCI |
原文出处 | http://pubs.acs.org/doi/abs/10.1021/ac403462f |
语种 | 英语 |
内容类型 | 期刊论文 |
源URL | [http://ir.siat.ac.cn:8080/handle/172644/5157] ![]() |
专题 | 深圳先进技术研究院_医药所 |
作者单位 | ANALYTICAL CHEMISTRY |
推荐引用方式 GB/T 7714 | Li, Ying,Liang, Li,Zhang, Chun-yang. Isothermally sensitive detection of serum circulating miRNAs for lung cancer diagnosis[J]. ANALYTICAL CHEMISTRY,2013. |
APA | Li, Ying,Liang, Li,&Zhang, Chun-yang.(2013).Isothermally sensitive detection of serum circulating miRNAs for lung cancer diagnosis.ANALYTICAL CHEMISTRY. |
MLA | Li, Ying,et al."Isothermally sensitive detection of serum circulating miRNAs for lung cancer diagnosis".ANALYTICAL CHEMISTRY (2013). |
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