Isothermally sensitive detection of serum circulating miRNAs for lung cancer diagnosis

	Isothermally sensitive detection of serum circulating miRNAs for lung cancer diagnosis
	Li, Ying; Liang, Li; Zhang, Chun-yang
刊名	ANALYTICAL CHEMISTRY
	2013
英文摘要	Tumor-derived miRNAs in serum are emerging as the new noninvasive biomarkers for the diagnosis of human cancers, especially at their early stage. An ideal method with high sensitivity, excellent selectivity, a simple procedure, and small amounts of starting materials is imperative for the detection of cliniccirculating miRNAs. Here, we develop a new method for isothermally sensitive detection of serum miRNAs using hairpin probe-based rolling circle amplification (HP-RCA). This method exhibits ultrahigh sensitivity toward lung cancer-related miR-486-5p with a detection limit of as low as 10 fM and a large dynamic rangeof 6 orders of magnitude, and it can even discriminate miR-486-5p from both miRNAs with high sequence homology and its precursors (pre-miRNAs). More importantly, this method can directly and accurately distinguish the expression of serum miR-486-5p among six nonsmall-cell lung carcinoma (NSCLC) patients and six healthy persons, holding a great potential for further applications in the clinical diagnosis of lung cancers.
收录类别	SCI
原文出处	http://pubs.acs.org/doi/abs/10.1021/ac403462f
语种	英语
内容类型	期刊论文
源URL	[http://ir.siat.ac.cn:8080/handle/172644/5157]
专题	深圳先进技术研究院_医药所
作者单位	ANALYTICAL CHEMISTRY
推荐引用方式 GB/T 7714	Li, Ying,Liang, Li,Zhang, Chun-yang. Isothermally sensitive detection of serum circulating miRNAs for lung cancer diagnosis[J]. ANALYTICAL CHEMISTRY,2013.
APA	Li, Ying,Liang, Li,&Zhang, Chun-yang.(2013).Isothermally sensitive detection of serum circulating miRNAs for lung cancer diagnosis.ANALYTICAL CHEMISTRY.
MLA	Li, Ying,et al."Isothermally sensitive detection of serum circulating miRNAs for lung cancer diagnosis".ANALYTICAL CHEMISTRY (2013).