Real-time detection of transcription factors using target-converted helicase-dependent amplification assay with zero-background signal

	Real-time detection of transcription factors using target-converted helicase-dependent amplification assay with zero-background signal
	Cao, Anping; Zhang, Chun-yang
刊名	ANALYTICAL CHEMISTRY
	2013
英文摘要	Highly sensitive detection of transcription factors is essential to the evaluation of cellular development and the disease state. However, so far most detectionmethods are usually laborious and time-consuming with a poor sensitivity. Here, we demonstrate a simple and ultrasensitive approach for transcription factordetection based on the target-converted helicase-dependent amplification assay. We employ a hairpin probe bearing the transcription factor binding site to convert the protein signal to the DNA signal, which can be further amplified by helicase-dependent amplification. With the digestion of excess probes by the exonucleases and the subsequent one primer-triggered high fidelity amplification, zero-background signal can be achieved in the absence of a transcriptionfactor. This method exhibits excellent specificity and high sensitivity with the detection limit of 9.3x 10(-13) M and the detection range over 4 orders ofmagnitude, which is superior to most currently used approaches for transcription factor detection. Moreover, the proposed method has significant advantages ofsimple, rapid, and low cost without the need of any labeled DNA probes and might be extended to selectively detect various DNA-binding proteins by simply changing the binding-site sequences of hairpin probes.
收录类别	SCI
原文出处	http://pubs.acs.org/doi/abs/10.1021/ac400010r
语种	英语
内容类型	期刊论文
源URL	[http://ir.siat.ac.cn:8080/handle/172644/5153]
专题	深圳先进技术研究院_医药所
作者单位	ANALYTICAL CHEMISTRY
推荐引用方式 GB/T 7714	Cao, Anping,Zhang, Chun-yang. Real-time detection of transcription factors using target-converted helicase-dependent amplification assay with zero-background signal[J]. ANALYTICAL CHEMISTRY,2013.
APA	Cao, Anping,&Zhang, Chun-yang.(2013).Real-time detection of transcription factors using target-converted helicase-dependent amplification assay with zero-background signal.ANALYTICAL CHEMISTRY.
MLA	Cao, Anping,et al."Real-time detection of transcription factors using target-converted helicase-dependent amplification assay with zero-background signal".ANALYTICAL CHEMISTRY (2013).