PEI protected aptamer molecular probes for contrast-enhanced in vivo cancer imaging

	PEI protected aptamer molecular probes for contrast-enhanced in vivo cancer imaging
	Gong, Ping; Shi, Bihua; Zheng, Mingbin; Wang, Bi; Zhang, Pengfei; Hu, Dehong; Gao, Duyang; Sheng, Zonghai; Zheng, Cuifang; Ma, Yifan
刊名	BIOMACROMOLECULES
	2012
英文摘要	Aptamers have emerged as promising molecular probes for cancer diagnosis. However, their application for in vivo cancer imaging remains limitation due to the poor stability in blood and the degradation by nucleases. In the present study, we generated PEI/aptamer molecular complexes for cancer imaging in vivo by using deoxyribonuclease (DNase)-activatable fluorescence probes (DFProbes) to monitor DNA degradation. The results showed that the complexes with PEI at the N/P ratio from 3.8 to 15 effectively prevented the degradation of DEProbes both in vitro and in vivo. Moreover, PEI successfully protected TD05 aptamers from DNase degradation without affecting its specific recognition of Ramos cells. In tumor bearing mice, PEI/aptamer molecular complexes further demonstrated superior passive tumor targeting and extended circulation time as compared with free aptamer. Hence, the well-defined PEI/aptamer probe is a novel strategy to deliver targeted aptamer for tumor diagnosis and imaging in vivo. (c) 2012 Elsevier Ltd. All rights reserved.
收录类别	SCI
原文出处	http://www.sciencedirect.com/science/article/pii/S0142961212007806
语种	英语
内容类型	期刊论文
源URL	[http://ir.siat.ac.cn:8080/handle/172644/4286]
专题	深圳先进技术研究院_医药所
作者单位	BIOMACROMOLECULES
推荐引用方式 GB/T 7714	Gong, Ping,Shi, Bihua,Zheng, Mingbin,et al. PEI protected aptamer molecular probes for contrast-enhanced in vivo cancer imaging[J]. BIOMACROMOLECULES,2012.
APA	Gong, Ping.,Shi, Bihua.,Zheng, Mingbin.,Wang, Bi.,Zhang, Pengfei.,...&Cai, Lintao.(2012).PEI protected aptamer molecular probes for contrast-enhanced in vivo cancer imaging.BIOMACROMOLECULES.
MLA	Gong, Ping,et al."PEI protected aptamer molecular probes for contrast-enhanced in vivo cancer imaging".BIOMACROMOLECULES (2012).