Rapid and label-free monitoring of exonuclease III-assisted target recycling amplification

	Rapid and label-free monitoring of exonuclease III-assisted target recycling amplification
	Xu, Qinfeng; Cao, Anping; Zhang, Ling-fei; Zhang, Chun-yang
刊名	ANALYTICAL CHEMISTRY
	2012
英文摘要	Target recycling-oriented amplification has been widely applied for sensitive detection of DNA, RNA, and proteins due to its successful overcoming the inherent limitation of target-to-signal ratio of 1:1 in the traditional hybridization assay. Exonuclease III (Exo III) is usually used as the cleavage enzyme in the targetrecycling-oriented amplification because of its easy availability, high catalytic activity, and wide applicability. Even though Exo III is assumed to be double-stranded DNA (dsDNA) specific exonuclease in most literature, its cleavage of single-strand DNA (ssDNA) does occur, resulting in the target-independent degradation of probes. Herein, we design an intramolecular displacement probe with the capability of resistance to the nonspecific digestion of Exo III and fast hybridization kinetics. Through the substitution of 2-aminopurine for adenine in the intramolecular displacement probes, we develop a rapid and label-freeapproach to monitor Exo III-assisted target recycling amplification. We further demonstrate that this method can be used for the detection of DNA and proteins with excellent specificity and high sensitivity. Importantly, this method can be extended to rapid, label-free and multiplexed detection of various nucleic acids, proteins, and small molecules using different kinds of fluorescent nucleotide analogues and specific aptamers.
收录类别	SCI
原文出处	http://pubs.acs.org/doi/abs/10.1021/ac303095z
语种	英语
内容类型	期刊论文
源URL	[http://ir.siat.ac.cn:8080/handle/172644/4280]
专题	深圳先进技术研究院_医药所
作者单位	ANALYTICAL CHEMISTRY
推荐引用方式 GB/T 7714	Xu, Qinfeng,Cao, Anping,Zhang, Ling-fei,et al. Rapid and label-free monitoring of exonuclease III-assisted target recycling amplification[J]. ANALYTICAL CHEMISTRY,2012.
APA	Xu, Qinfeng,Cao, Anping,Zhang, Ling-fei,&Zhang, Chun-yang.(2012).Rapid and label-free monitoring of exonuclease III-assisted target recycling amplification.ANALYTICAL CHEMISTRY.
MLA	Xu, Qinfeng,et al."Rapid and label-free monitoring of exonuclease III-assisted target recycling amplification".ANALYTICAL CHEMISTRY (2012).