Highly sensitive detection of protein with aptamer-based target-triggering two-stage amplification

	Highly sensitive detection of protein with aptamer-based target-triggering two-stage amplification
	Zhang, Zhen-zhu; Zhang, Chun-yang
刊名	ANALYTICAL CHEMISTRY
	2012
英文摘要	Highly sensitive detection of proteins is essential to biomedical research as well as clinical diagnosis. However, so far most detection methods rely on antibody-based assays and are usually laborious and time-consuming with poor sensitivity. Here, we develop a simple and sensitive method for the detection ofa biomarker protein, platelet-derived growth factor BB (PDGF-BB), based on aptamer-based target-triggering two-stage amplification. With the involvement of anaptamer-based probe and an exponential amplification reaction (EXPAR) template, our method combines strand displacement amplification (SDA) and EXPAR, transforming the probe conformational change induced by target binding into two-stage amplification and distinct fluorescence signal. This detection method exhibits excellent specificity and high sensitivity with a detection limit of 9.04 X 10(-13) M and a detection range of more than 5 orders of magnitude, which is comparable with or even superior to most currently used approaches for PDGF-BB detection. Moreover, this detection method has significant advantages ofisothermal conditions required, simple and rapid without multiple separation and washing steps, low-cost without the need of any labeled DNA probes. Furthermore, this method might be extended to sensitive detection of a variety of biomolecules whose aptamers undergo similar conformational changes.
收录类别	SCI
原文出处	http://pubs.acs.org/doi/abs/10.1021/ac2029002
语种	英语
内容类型	期刊论文
源URL	[http://ir.siat.ac.cn:8080/handle/172644/4279]
专题	深圳先进技术研究院_医药所
作者单位	ANALYTICAL CHEMISTRY
推荐引用方式 GB/T 7714	Zhang, Zhen-zhu,Zhang, Chun-yang. Highly sensitive detection of protein with aptamer-based target-triggering two-stage amplification[J]. ANALYTICAL CHEMISTRY,2012.
APA	Zhang, Zhen-zhu,&Zhang, Chun-yang.(2012).Highly sensitive detection of protein with aptamer-based target-triggering two-stage amplification.ANALYTICAL CHEMISTRY.
MLA	Zhang, Zhen-zhu,et al."Highly sensitive detection of protein with aptamer-based target-triggering two-stage amplification".ANALYTICAL CHEMISTRY (2012).