A bright cyan-excitable orange fluorescent protein facilitates dual-emission microscopy and enhances bioluminescence imaging in vivo

	A bright cyan-excitable orange fluorescent protein facilitates dual-emission microscopy and enhances bioluminescence imaging in vivo
	J. Chu; Y. Oh; A. Sens; N. Ataie; H. Dana; J. J. Macklin; T. Laviv; E. S. Welf; K. M. Dean; F. Zhang
刊名	Nature Biotechnology
	2016
英文摘要	Orange-red fluorescent proteins (FPs) are widely used in biomedical research for multiplexed epifluorescence microscopy with GFP-based probes, but their different excitation requirements make multiplexing with new advanced microscopy methods difficult. Separately, orange-red FPs are useful for deep-tissue imaging in mammals owing to the relative tissue transmissibility of orange-red light, but their dependence on illumination limits their sensitivity as reporters in deep tissues. Here we describe CyOFP1, a bright, engineered, orange-red FP that is excitable by cyan light. We show that CyOFP1 enables single-excitation multiplexed imaging with GFP-based probes in single-photon and two-photon microscopy, including time-lapse imaging in light-sheet systems. CyOFP1 also serves as an efficient acceptor for resonance energy transfer from the highly catalytic blue-emitting luciferase NanoLuc. An optimized fusion of CyOFP1 and NanoLuc, called Antares, functions as a highly sensitive bioluminescent reporter in vivo, producing substantially brighter signals from deep tissues than firefly luciferase and other bioluminescent proteins.
收录类别	SCI
原文出处	http://www.nature.com/nbt/journal/v34/n7/full/nbt.3550.html
语种	英语
内容类型	期刊论文
源URL	[http://ir.siat.ac.cn:8080/handle/172644/10503]
专题	深圳先进技术研究院_医工所
作者单位	Nature Biotechnology
推荐引用方式 GB/T 7714	J. Chu,Y. Oh,A. Sens,et al. A bright cyan-excitable orange fluorescent protein facilitates dual-emission microscopy and enhances bioluminescence imaging in vivo[J]. Nature Biotechnology,2016.
APA	J. Chu.,Y. Oh.,A. Sens.,N. Ataie.,H. Dana.,...&M. Lin.(2016).A bright cyan-excitable orange fluorescent protein facilitates dual-emission microscopy and enhances bioluminescence imaging in vivo.Nature Biotechnology.
MLA	J. Chu,et al."A bright cyan-excitable orange fluorescent protein facilitates dual-emission microscopy and enhances bioluminescence imaging in vivo".Nature Biotechnology (2016).