A LIPOPEPTIDE-BASED alpha V beta(3) INTEGRIN-TARGETED ULTRASOUND CONTRAST AGENT FOR MOLECULAR IMAGING OF TUMOR ANGIOGENESIS

	A LIPOPEPTIDE-BASED alpha V beta(3) INTEGRIN-TARGETED ULTRASOUND CONTRAST AGENT FOR MOLECULAR IMAGING OF TUMOR ANGIOGENESIS
	Yan, Fei; Xu, Xiuxia; Chen, Yihan; Deng, Zhiting; Liu, Hongmei; Xu, Jianrong; Zhou, Jie; Tan, Guanghong; Wu, Junru; Zheng, Hairong
刊名	ULTRASOUND IN MEDICINE AND BIOLOGY
	2015
英文摘要	The design and fabrication of targeted ultrasound contrast agents are key factors in the success of ultrasound molecular imaging applications. Here, we introduce a transformable alpha v beta(3) integrin-targeted microbubble (MB) by incorporation of iRGD-lipopeptides into the MB membrane for non-invasive ultrasound imaging of tumor angiogenesis. First, the iRGD-lipopeptides were synthesized by conjugating iRGD peptides to distearoyl-phosphatidylethanolamine-polyethylene glycol 2000-maleimide. The resulting iRGD-lipopeptides were used for fabrication of the iRGD-carrying alpha vbeta(3) integrin-targeted MBs (iRGD-MBs). The binding specificity of iRGDMBs for endothelial cells was found to be significantly stronger than that ofcontrol MBs (p < 0.01) under in vitro static and dynamic conditions. The binding of iRGD-MBs on the endothelial cells was competed off by pre-incubation with the anti-alpha v or anti-beta(3) antibody (p < 0.01). Ultrasound images taken of mice bearing 4T1 breast tumors after intravenous injections of iRGD-MBs or control MBs revealed strong contrast enhancement within the tumors from iRGD-MBs but not from the control MBs; the mean acoustic signal intensity was 10.71 +/- 2.75 intensity units for iRGD-MBs versus 1.13 +/- 0.18 intensity units for the control MBs (p, 0.01). The presenceof alpha v beta(3) integrin was confirmed by immunofluorescence staining. These data indicate that iRGD-MBs can be used as an ultrasound imagingprobe for the non-invasive molecular imaging of tumor angiogenesis, and may have further implications for ultrasound image-guided tumor targeting drug delivery.
收录类别	SCI
原文出处	http://www.sciencedirect.com/science/article/pii/S0301562915003968
语种	英语
WOS记录号	WOS:000366470400022
内容类型	期刊论文
源URL	[http://ir.siat.ac.cn:8080/handle/172644/7205]
专题	深圳先进技术研究院_医工所
作者单位	ULTRASOUND IN MEDICINE AND BIOLOGY
推荐引用方式 GB/T 7714	Yan, Fei,Xu, Xiuxia,Chen, Yihan,et al. A LIPOPEPTIDE-BASED alpha V beta(3) INTEGRIN-TARGETED ULTRASOUND CONTRAST AGENT FOR MOLECULAR IMAGING OF TUMOR ANGIOGENESIS[J]. ULTRASOUND IN MEDICINE AND BIOLOGY,2015.
APA	Yan, Fei.,Xu, Xiuxia.,Chen, Yihan.,Deng, Zhiting.,Liu, Hongmei.,...&Zheng, Hairong.(2015).A LIPOPEPTIDE-BASED alpha V beta(3) INTEGRIN-TARGETED ULTRASOUND CONTRAST AGENT FOR MOLECULAR IMAGING OF TUMOR ANGIOGENESIS.ULTRASOUND IN MEDICINE AND BIOLOGY.
MLA	Yan, Fei,et al."A LIPOPEPTIDE-BASED alpha V beta(3) INTEGRIN-TARGETED ULTRASOUND CONTRAST AGENT FOR MOLECULAR IMAGING OF TUMOR ANGIOGENESIS".ULTRASOUND IN MEDICINE AND BIOLOGY (2015).