Size-Controlled Construction of Magnetic Nanoparticle Clusters Using DNA-Binding Zinc Finger Protein

	Size-Controlled Construction of Magnetic Nanoparticle Clusters Using DNA-Binding Zinc Finger Protein
	Yiseul Ryu; Zongwen Jin; Joong-jae Lee; Seung-hyun Noh; Tae-Hyun Shin; Seong-Min Jo; Joonsung Choi; HyunWook Park; Jinwoo Cheon; and Hak-Sung Kim
刊名	ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
	2015
英文摘要	Nanoparticle clusters (NPCs) have attracted significant interest owing to their unique characteristics arising from their collective individual properties. Nonetheless, the construction of NPCs in a structurally well-defined and sizecontrollable manner remains a challenge. Here we demonstrate a strategy to construct size-controlled NPCs using the DNAbinding zinc finger (ZnF) protein. Biotinylated ZnF was conjugated to DNA templates with different lengths, followed by incubation with neutravidin-conjugated nanoparticles. The sequence specificity of ZnF and programmable DNA templates enabled a size-controlled construction of NPCs, resulting in a homogeneous size distribution. We demonstrated the utility of magnetic NPCs by showing a three-fold increase in the spin–spin relaxivity in MRI compared with Feridex. Furthermore, folate-conjugated magnetic NPCs exhibited a specific targeting ability for HeLa cells. The present approach can be applicable to other nanoparticles, finding wide applications in many areas such as disease diagnosis, imaging, and delivery of drugs and genes.
收录类别	SCI
原文出处	http://onlinelibrary.wiley.com/doi/10.1002/anie.201408593/full
语种	英语
内容类型	期刊论文
源URL	[http://ir.siat.ac.cn:8080/handle/172644/7039]
专题	深圳先进技术研究院_医工所
作者单位	ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
推荐引用方式 GB/T 7714	Yiseul Ryu,Zongwen Jin,Joong-jae Lee,et al. Size-Controlled Construction of Magnetic Nanoparticle Clusters Using DNA-Binding Zinc Finger Protein[J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,2015.
APA	Yiseul Ryu.,Zongwen Jin.,Joong-jae Lee.,Seung-hyun Noh.,Tae-Hyun Shin.,...&and Hak-Sung Kim.(2015).Size-Controlled Construction of Magnetic Nanoparticle Clusters Using DNA-Binding Zinc Finger Protein.ANGEWANDTE CHEMIE-INTERNATIONAL EDITION.
MLA	Yiseul Ryu,et al."Size-Controlled Construction of Magnetic Nanoparticle Clusters Using DNA-Binding Zinc Finger Protein".ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2015).