Targeted Microbubble Mediated Sonoporation of Endothelial Cells In Vivo

	Targeted Microbubble Mediated Sonoporation of Endothelial Cells In Vivo
	Skachkov Ilya; Luan Ying; van der Steen Antonius F. W.; de Jong Nico; Kooiman Klazina
刊名	IEEE TRANSACTIONS ON ULTRASONICS FERROELECTRICS AND FREQUENCY CONTROL
	2014
英文摘要	Ultrasound contrast agents as drug-delivery systems are an emerging field. Recently, we reported that targeted microbubbles are able to sonoporate endothelial cells in vitro. In this study, we investigated whether targeted microbubbles can also induce sonoporation of endothelial cells in vivo, thereby making it possible to combine molecular imaging and drug delivery. Live chicken embryos were chosen as the in vivo model. αvß3-targeted microbubbles attached to the vessel wall of the chicken embryo were insonified at 1 MHz at 150 kPa (1 × 10 000 cycles) and at 200 kPa (1 × 1000 cycles) peak negative acoustic pressure. Sonoporation was studied by intravital microscopy using the model drug propidium iodide (PI). Endothelial cell PI uptake was observed in 48% of microbubble-vessel-wall complexes at 150 kPa (n = 140) and in 33% at 200 kPa (n = 140). Efficiency of PI uptake depended on the local targeted microbubble concentration and increased up to 80% for clusters of 10 to 16 targeted microbubbles. Ultrasound or targeted microbubbles alone did not induce PI uptake. This intravital microscopy study reveals that sonoporation can be visualized and induced in vivo using targeted microbubbles.
收录类别	SCI
原文出处	http://ieeexplore.ieee.org/xpl/articleDetails.jsp?arnumber=6910377
语种	英语
内容类型	期刊论文
源URL	[http://ir.siat.ac.cn:8080/handle/172644/5767]
专题	深圳先进技术研究院_医工所
作者单位	IEEE TRANSACTIONS ON ULTRASONICS FERROELECTRICS AND FREQUENCY CONTROL
推荐引用方式 GB/T 7714	Skachkov Ilya,Luan Ying,van der Steen Antonius F. W.,et al. Targeted Microbubble Mediated Sonoporation of Endothelial Cells In Vivo[J]. IEEE TRANSACTIONS ON ULTRASONICS FERROELECTRICS AND FREQUENCY CONTROL,2014.
APA	Skachkov Ilya,Luan Ying,van der Steen Antonius F. W.,de Jong Nico,&Kooiman Klazina.(2014).Targeted Microbubble Mediated Sonoporation of Endothelial Cells In Vivo.IEEE TRANSACTIONS ON ULTRASONICS FERROELECTRICS AND FREQUENCY CONTROL.
MLA	Skachkov Ilya,et al."Targeted Microbubble Mediated Sonoporation of Endothelial Cells In Vivo".IEEE TRANSACTIONS ON ULTRASONICS FERROELECTRICS AND FREQUENCY CONTROL (2014).