Efficacy of MRI visible iron oxide nanoparticles in delivering minicircle DNA into liver via intrabiliary infusion

	Efficacy of MRI visible iron oxide nanoparticles in delivering minicircle DNA into liver via intrabiliary infusion
	Lin Gao; Lisi Xie; Xiaojing Long; Zhiyong Wang; Cheng-Yi He; Zhi-Ying Chen; Lei Zhang; Xiang Nan; Hulong Lei; Xin Liu
刊名	BIOMATERIALS
	2013
英文摘要	Gene therapy is a very promising technology for treatment of liver diseases. Minicircle DNA (MCDNA) is a versatile gene vector, which possesses excellent features in bio-safety and duration of transgene expression. However, its application has been hampered by the lack of an efficient gene delivery system. In this study, we developed a magnetic resonance imaging (MRI) visible nanoparticle to monitor MCDNA gene delivery and explore the potential of gene therapy in vivo. The nanoparticle was constructed via self-assembly of stearic acid modified low molecular polyethyleneimine (stPEI) and superparamagnetic iron oxide (SPIO) nanocrystals. The multiple SPIO nanocrystals with a controlled clustering structure in the nanoparticles were designed to achieve high MRI sensitivity. Furthermore, the MCDNA was combined with the nanoparticles via electronic interaction. Through intrabiliary infusion, the stPEI-SPIO/MCDNA nanoparticles were efficiently delivered to liver that was visualized by MRI in vivo and confirmed by histology. Moreover, the MCDNA nanoparticles exhibited non-cytotoxicity with no obvious inflammation in the transfection sites. These results indicate that stPEI-SPIO nanoparticle is able to serve as both an efficient DNA vector delivery system and a sensitive agent for MRI visualization.
收录类别	SCI
原文出处	http://www.sciencedirect.com/science/article/pii/S014296121300149X
语种	英语
内容类型	期刊论文
源URL	[http://ir.siat.ac.cn:8080/handle/172644/4751]
专题	深圳先进技术研究院_医工所
作者单位	BIOMATERIALS
推荐引用方式 GB/T 7714	Lin Gao,Lisi Xie,Xiaojing Long,et al. Efficacy of MRI visible iron oxide nanoparticles in delivering minicircle DNA into liver via intrabiliary infusion[J]. BIOMATERIALS,2013.
APA	Lin Gao.,Lisi Xie.,Xiaojing Long.,Zhiyong Wang.,Cheng-Yi He.,...&Bensheng Qiu.(2013).Efficacy of MRI visible iron oxide nanoparticles in delivering minicircle DNA into liver via intrabiliary infusion.BIOMATERIALS.
MLA	Lin Gao,et al."Efficacy of MRI visible iron oxide nanoparticles in delivering minicircle DNA into liver via intrabiliary infusion".BIOMATERIALS (2013).