Exogenous MC3T3 Pre-osteoblasts Migrate Systemically and Mitigate the Adverse Effects of Wear Particles

	Exogenous MC3T3 Pre-osteoblasts Migrate Systemically and Mitigate the Adverse Effects of Wear Particles
	Kate Fritton, M.S.; Pei-Gen Ren, D.V.M.; Emmanuel Gibon; Allison J. Rao; Ting Ma; Sandip Biswal; Sanjiv S. Gambhir; Stuart B. Goodman
刊名	TISSUE ENGINEERING
	2012
英文摘要	Understanding how relevant cell types respond to wear particles will reveal new avenues for treating osteolysis following joint replacements. In this study, we investigate the effects of ultrahigh molecular weight polyethylene (UHMWPE) particles on preosteoblast migration and function. We infused UHMWPE particles or saline into the left femur of mice and injected luciferase-expressing preosteoblasts (MC3T3 cells) into each left ventricle. Bioluminescence imaging (BLI) confirmed systemic administration of MC3T3 cells. BLI throughout the 28-day experiment showed greater MC3T3 migration to the site of particle infusion than to the site of saline infusion, with significant differences on days 0, 4, and 6 (p≤0.055). Immunostaining revealed a greater number of osteoblasts and osteoclasts in the particle-infused femora, indicating greater bone turnover. The bone mineralization of the particle-infused femora increased significantly when compared to saline-infused femora (an increase of 146.4±27.9 vs. 12.8±8.7 mg/mL, p=0.008). These results show that infused preosteoblasts can migrate to the site of wear particles. Additionally, as the migrated cells were associated with increased bone mineralization in spite of the presence of particles, increasing osteoblast recruitment is a potential strategy for combating bone loss due to increased osteoclast/macrophage number and decreased osteoblast function.
收录类别	SCI
原文出处	http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3501117/pdf/ten.tea.2012.0086.pdf
语种	英语
内容类型	期刊论文
源URL	[http://ir.siat.ac.cn:8080/handle/172644/4022]
专题	深圳先进技术研究院_医工所
作者单位	TISSUE ENGINEERING
推荐引用方式 GB/T 7714	Kate Fritton, M.S.,Pei-Gen Ren, D.V.M.,Emmanuel Gibon,et al. Exogenous MC3T3 Pre-osteoblasts Migrate Systemically and Mitigate the Adverse Effects of Wear Particles[J]. TISSUE ENGINEERING,2012.
APA	Kate Fritton, M.S..,Pei-Gen Ren, D.V.M..,Emmanuel Gibon.,Allison J. Rao.,Ting Ma.,...&Stuart B. Goodman.(2012).Exogenous MC3T3 Pre-osteoblasts Migrate Systemically and Mitigate the Adverse Effects of Wear Particles.TISSUE ENGINEERING.
MLA	Kate Fritton, M.S.,et al."Exogenous MC3T3 Pre-osteoblasts Migrate Systemically and Mitigate the Adverse Effects of Wear Particles".TISSUE ENGINEERING (2012).