Feature-based Automatic Real-time Tumor Motion Monitoring With or Without Fiducial Markers

	Feature-based Automatic Real-time Tumor Motion Monitoring With or Without Fiducial Markers
	Wu Liu; Yaoqin Xie; Wei-yang Lin; Gary Luxton; Ravinder Nat
刊名	INT J RADIAT ONCOL
	2012
英文摘要	Suicide gene therapy has become an effective therapy for breast cancer, and ultrasound targeted microbubble destruction (UTMD) has become a popular topic in the gene therapy field. In this study, MCF-7 cells with the KDR promoter and LSl74T cells without the KDR promoter were transfected with the recombinant plasmid pEGFP-KDRP-CD/TK using UTMD. The recombinant plasmid pEGFP-KDRP-CD/TK was transfected into MCF-7 and LS174T cells successfully with no significant difference in transfection efficiency (p > 0.05). By RT-PCR, the CD/TK fusion gene was shown to be expressed in MCF-7 cells but not expressed in LS174T cells. In a cytotoxicity experiment, transgenic MCF-7 cells were sensitive to the prodrugs 5-FC and GCV. When both 5-FC and GCV were administered, the rate of cellular inhibition was significantly greater than that achieved when only one of the prodrugs was administered (p < 0.001). Moreover, the inhibition rates achieved administering 5-FC, GCV and both 5-FC and GCV were all significantly greater than the gene transfection rate of 21.92 +/- 3.64% (p < 0.001). However, transgenic LS174T cells were not sensitive to any prodrug. These results demonstrated that UTMD is a safe, effective and targeted gene delivery system. Also, the KDR promoter can drive expression of the CD/TK double suicide gene target in MCF-7 cells, and the targeted killing effect of the KDRP-CD/TK gene on MCF-7 cells in vitro has good synergy with expression of the CD/TK fusion gene. (C) 2011 Elsevier B.V. All rights reserved
收录类别	SCI
原文出处	http://astro2012.abstractsnet.com/handouts/011470_ASTRO2012_Epost.pdf
语种	英语
内容类型	期刊论文
源URL	[http://ir.siat.ac.cn:8080/handle/172644/4004]
专题	深圳先进技术研究院_医工所
作者单位	INT J RADIAT ONCOL
推荐引用方式 GB/T 7714	Wu Liu,Yaoqin Xie,Wei-yang Lin,et al. Feature-based Automatic Real-time Tumor Motion Monitoring With or Without Fiducial Markers[J]. INT J RADIAT ONCOL,2012.
APA	Wu Liu,Yaoqin Xie,Wei-yang Lin,Gary Luxton,&Ravinder Nat.(2012).Feature-based Automatic Real-time Tumor Motion Monitoring With or Without Fiducial Markers.INT J RADIAT ONCOL.
MLA	Wu Liu,et al."Feature-based Automatic Real-time Tumor Motion Monitoring With or Without Fiducial Markers".INT J RADIAT ONCOL (2012).