A novel formulation of lipid microbubbles coated with stearic acid modified polyethylenimine to enhance ultrasound-mediated gene delivery in vitro

	A novel formulation of lipid microbubbles coated with stearic acid modified polyethylenimine to enhance ultrasound-mediated gene delivery in vitro
	Qiaofeng Jin; Zhiyong Wang; Fei Yan; Zhiting Deng; Lu Li; Hairong Zheng
刊名	The Journal of the Acoustical Society of America
	2012
英文摘要	The aim of this study was to assess the properties of a new designed cationic microbubbles as gene carriers and the relative gene transfection efficacy with ultrasound triggered microbubble destruction. Polyethylenimine as a high efficient gene transfection agent has higher cell toxicity with molecular weight increasing. Stearic acid was used to modify branched polyethylenimine to change its hydrophilic properties so that it can be assembled onto the lipid shell of the microbubbles and simultaneously decrease its cell toxicity. Cationic microbubbles was prepared by encapsulating perfluoropropane into phospholipids and stearic acid modified polyethylenimine hybrid shell using mechanical vibration method. The mean, median size and zeta potential of the microbubbles were measured 1.841.62um, 1.60um and 54mv respectively. Hoechst 33258 was used to stain the green fluorescent protein reporter plasmid which was charge-coupled to cationic microbubbles, and microbubbles was observed emitting blue light under a fluorescence microscope. About 4ug plasmid loaded by 10(8) microbubles that contain 5% mole ratio stearic acid modified polyethylenimine was measured by gel electrophoresis. A 1.25MHz single element transducer was used to mediate the gene transfection to MCF-7 cell by using the cationic microbubbles and enhancement of green fluorescent protein expression was observed. A novel formulation of lipid microbubbles coated with stearic acid modified polyethylenimine to enhance ultrasound-mediated gene delivery in vitro. - ResearchGate. Available from: http://www.researchgate.net/publication/224035653_A_novel_formulation_of_lipid_microbubbles_coated_with_stearic_acid_modified_polyethylenimine_to_enhance_ultrasound-mediated_gene_delivery_in_vitro [accessed Jun 5, 2015].
收录类别	其他
原文出处	http://scitation.aip.org/content/asa/journal/jasa/131/4/10.1121/1.4708688
语种	英语
内容类型	期刊论文
源URL	[http://ir.siat.ac.cn:8080/handle/172644/3958]
专题	深圳先进技术研究院_医工所
作者单位	The Journal of the Acoustical Society of America
推荐引用方式 GB/T 7714	Qiaofeng Jin,Zhiyong Wang,Fei Yan,et al. A novel formulation of lipid microbubbles coated with stearic acid modified polyethylenimine to enhance ultrasound-mediated gene delivery in vitro[J]. The Journal of the Acoustical Society of America,2012.
APA	Qiaofeng Jin,Zhiyong Wang,Fei Yan,Zhiting Deng,Lu Li,&Hairong Zheng.(2012).A novel formulation of lipid microbubbles coated with stearic acid modified polyethylenimine to enhance ultrasound-mediated gene delivery in vitro.The Journal of the Acoustical Society of America.
MLA	Qiaofeng Jin,et al."A novel formulation of lipid microbubbles coated with stearic acid modified polyethylenimine to enhance ultrasound-mediated gene delivery in vitro".The Journal of the Acoustical Society of America (2012).