ULTRASONIC IMAGING OF ENDOTHELIAL CD81 EXPRESSION USING CD81-TARGETED CONTRAST AGENTS IN IN VITRO AND IN VIVO STUDIES

	ULTRASONIC IMAGING OF ENDOTHELIAL CD81 EXPRESSION USING CD81-TARGETED CONTRAST AGENTS IN IN VITRO AND IN VIVO STUDIES
	FEI YAN; XIANG LI; QIAOFENG JIN; JUANJUAN CHEN; ROBIN SHANDAS; JUNRU WU; LU LI; TAO LING; WEI YANG; YUN CHEN
刊名	ULTRASOUND IN MEDICINE AND BIOLOGY
	2012
英文摘要	This study is designed to investigate the feasibility for molecular imaging of endothelial CD81 expression in vitro and in vivo using the CD81-targeted ultrasound contrast agents (UCA). In the in vitro study, murine bEnd.3 cells were stimulated with phenazine methosulfate (PMS), an oxidative stress inducer. Changes in CD81 expression after stimulation were confirmed by Western blotting, tracked by using the targeted UCA and further imaged under ultrasound imaging system with 5 MHz transmit frequency. In the in vivo study, expression of endothelial CD81 proteins in murine carotid artery vessels was studied using high-frequency ultrasound system with 40 MHz transmit frequency. Our results showed that endothelial CD81 expression was gradually up-regulated with the increase of PMS concentration. Correspondingly, the accumulation of targeted UCA was gradually improved and could be inhibited significantly upon addition of free anti-CD81 antibodies. The mean video intensity (grey-level) of stimulated cells and vessels from backscatter of the CD81-targeted UCA was 17.2 (interquartile range [IQR] 15.4-19.8) and 27.2 (IQR 22.4-29.8), significantly greater than that of non-stimulated cells with 9.0 (IQR 8.6-10.8) (p < 0.01) and non-stimulated vessels with 11.3 (IQR 10.4-13.2) (p < 0.01), respectively. In conclusion, CD81-targeted UCA allows noninvasive assessment of the expression levels of CD81 on the vascular endothelium and may provide potential insights into early atherosclerotic plaque detection and treatment monitoring.
收录类别	SCI
原文出处	http://ac.els-cdn.com/S0301562912000087/1-s2.0-S0301562912000087-main.pdf?_tid=74e4daba-084a-11e5-8b72-00000aab0f02&acdnat=1433155338_2fa1f4270151811f47b68b8a5bce23c0
语种	英语
内容类型	期刊论文
源URL	[http://ir.siat.ac.cn:8080/handle/172644/3949]
专题	深圳先进技术研究院_医工所
作者单位	ULTRASOUND IN MEDICINE AND BIOLOGY
推荐引用方式 GB/T 7714	FEI YAN,XIANG LI,QIAOFENG JIN,et al. ULTRASONIC IMAGING OF ENDOTHELIAL CD81 EXPRESSION USING CD81-TARGETED CONTRAST AGENTS IN IN VITRO AND IN VIVO STUDIES[J]. ULTRASOUND IN MEDICINE AND BIOLOGY,2012.
APA	FEI YAN.,XIANG LI.,QIAOFENG JIN.,JUANJUAN CHEN.,ROBIN SHANDAS.,...&HAIRONG ZHENG.(2012).ULTRASONIC IMAGING OF ENDOTHELIAL CD81 EXPRESSION USING CD81-TARGETED CONTRAST AGENTS IN IN VITRO AND IN VIVO STUDIES.ULTRASOUND IN MEDICINE AND BIOLOGY.
MLA	FEI YAN,et al."ULTRASONIC IMAGING OF ENDOTHELIAL CD81 EXPRESSION USING CD81-TARGETED CONTRAST AGENTS IN IN VITRO AND IN VIVO STUDIES".ULTRASOUND IN MEDICINE AND BIOLOGY (2012).