Enriched environment and physical activity reduce microglia and influence the fate of NG2 cells in the amygdala of adult mice

	Enriched environment and physical activity reduce microglia and influence the fate of NG2 cells in the amygdala of adult mice
	Dan Ehninger; Li-Ping Wang; Friederike Klempin; Benedikt Römer; Helmut Kettenmann; Gerd Kempermann
刊名	CELL AND TISSUE RESEARCH
	2011
卷号	351 期号:1 页码:69-86
英文摘要	Proliferative cells expressing proteoglycan neuron-glia 2 (NG2) are considered to represent parenchymal precursor cells in the adult brain and are thought to differentiate primarily into oligodendrocytes. We have studied cell genesis in the adult amygdala and found that, up to 1 year after the labeling of proliferating cells with bromodeoxyuridine, most proliferating NG2 cells remain NG2 cells, and only a few slowly differentiate into mature oligodendrocytes, as assessed by the expression of 2',3'-cyclic nucleotide 3'-phosphodiesterase. We have detected no signs of neurogenesis but have confirmed the expression of “neuronal” markers such as Doublecortin in NG2 cells. Nestin-expressing NG2 cells in the amygdala show electrophysiological properties known for oligodendrocyte precursor cells in the corpus callosum. Application of the glutamate agonist kainate elicits a “complex” response consisting of a rapid and long-lasting blockade of the resting K+ conductance, a transient cationic current, and a transient increase of an outwardly directed K+ conductance, suggesting the responsiveness of NG2 cells to excitation. Proliferation of NG2 cells increases in response to behavioral stimuli of activity, voluntary wheel running, and environmental enrichment. In addition to reducing the number of newborn microglia, behavioral activity results in a decrease in S100β-expressing newborn NG2 cells in the amygdala. Because S100β expression in NG2 cells ceases with oligodendrocyte maturation, this finding suggests that NG2 cells in the amygdala undergo activity-dependent functional alterations, without resulting in a measurable increase in new mature oligodendrocytes over the time period covered by the present study. The adult amygdala thus shows signs of mixed activity-dependent plasticity: reduced numbers of microglia and, presumably, an altered fate of NG2 cells.
收录类别	SCI
原文出处	http://link.springer.com/article/10.1007/s00441-011-1200-z
语种	英语
内容类型	期刊论文
源URL	[http://ir.siat.ac.cn:8080/handle/172644/3470]
专题	深圳先进技术研究院_医工所
作者单位	CELL AND TISSUE RESEARCH
推荐引用方式 GB/T 7714	Dan Ehninger,Li-Ping Wang,Friederike Klempin,et al. Enriched environment and physical activity reduce microglia and influence the fate of NG2 cells in the amygdala of adult mice[J]. CELL AND TISSUE RESEARCH,2011,351(1):69-86.
APA	Dan Ehninger,Li-Ping Wang,Friederike Klempin,Benedikt Römer,Helmut Kettenmann,&Gerd Kempermann.(2011).Enriched environment and physical activity reduce microglia and influence the fate of NG2 cells in the amygdala of adult mice.CELL AND TISSUE RESEARCH,351(1),69-86.
MLA	Dan Ehninger,et al."Enriched environment and physical activity reduce microglia and influence the fate of NG2 cells in the amygdala of adult mice".CELL AND TISSUE RESEARCH 351.1(2011):69-86.