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	The Study of Healing Mechanism of Collagen-Induced Arthritis Rats Model Associated with Open Fracture
	Yiping Hu; Ling Qin; Yingya Ma; Wenxiang Cheng; Huang Huan; Jianhai Chen; Peng Zhang
	2016
会议名称	2016COA
会议地点	深圳
英文摘要	Objective: Patients with rheumatoid arthritis (RA) are at increased risk for developing osteoporosis and fractures compared to controls. This risk is further increased since a majority of patients with RA also receive concomitant heal difficult when fracture. In addition, multiple factors lead to systemic osteoporosis. These conditions cause decreased bone mineral density and bone quality, and expose patients to an increased risk of fracture. Methods: To observe the fracture heal in RA model, after 15 days the onset of arthritis a mid-shaft femur fracture model (RA+F group) was established (normal rats fracture model as control group NF group). Femurs were then harvested at 4 weeks, 8 weeks (n=5 per group) after fracture for micro- computed tomography (Micro-CT), X-Ray, hematoxylin and eosin staining (HE), toluidine blue staining and Safranin'O staining test. Results: The Micro CT images and HE staining demonstrated the remodeling of the calcified callus, at 4 and 8 weeks after fracture, fracture gap could be clearly observed in RA+F group, while NF group better heal than RA+F group. NF group and the periosteal callus appeared to be homogenously filled with newly formed bone. Bone mineral density, percent bone volume, trabecular thickness and trabecular number in the RA+F group was significantly inferior to the NF group at all the time points (P﹤0.05), simultaneously, we found structure model index test in the RA+F group was significantly higher than the NF group at all the time points (P﹤0.05). X-Ray observed the results same as Micro-CT images. Fracture gap clearly observed in RA+F group. Toluidine blue staining and Safranin'O staining test obtained from the NF group after fracture showed more mature lamellar bone in the fracture gap, with more integrity, thick and continuous callus compared to RA+F group. Conclusion: Multiple factors lead to systemic osteoporosis remarkably induced decrease in mineralized tissue and not well connected microstructure in newly formed callus, which may explain why RA patients has high risk of fracture and difficult heal when fracture.
收录类别	其他
语种	英语
内容类型	会议论文
源URL	[http://ir.siat.ac.cn:8080/handle/172644/10628]
专题	深圳先进技术研究院_医工所
作者单位	2016
推荐引用方式 GB/T 7714	Yiping Hu,Ling Qin,Yingya Ma,et al. The Study of Healing Mechanism of Collagen-Induced Arthritis Rats Model Associated with Open Fracture[C]. 见:2016COA. 深圳.

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