Intravital Microscopy of Localized Stem Cell Delivery Using Microbubbles and Acoustic Radiation Force

	Intravital Microscopy of Localized Stem Cell Delivery Using Microbubbles and Acoustic Radiation Force
	Kokhuis T. J. A.; Skachkov I.; Naaijkens B. A.; Juffermans L. J. M.; Kamp O.; Kooiman K.; van der Steen A. F. W; Versluis M.; de Jong N
刊名	BIOTECHNOLOGY AND BIOENGINEERING
	2015
英文摘要	The use of stem cells for the repair of damaged cardiac tissue after a myocardial infarction holds great promise. However, a common finding in experimental studies is the low number of cells delivered at the area at risk. To improve the delivery, we are currently investigating a novel delivery platform in which stem cells are conjugated with targeted microbubbles, creating echogenic complexes dubbed StemBells. These StemBells vibrate in response to incoming ultrasound waves making them susceptible to acoustic radiation force. The acoustic force can then be employed to propel circulating StemBells from the centerline of the vessel to the wall, facilitating localized stem cell delivery. In this study, we investigate the feasibility of manipulating StemBells acoustically in vivo after injection using a chicken embryo model. Bare stem cells or unsaturated stem cells (<5 bubbles/cell) do not respond to ultrasound application (1MHz, peak negative acoustical pressure P_ = 200 kPa, 10% duty cycle). However, stem cells which are fully saturated with targeted microbubbles (>30 bubbles/cell) can be propelled toward and arrested at the vessel wall. The mean translational velocities measured are 61 and 177 mu m/s for P_ = 200 and 450 kPa, respectively. This technique therefore offers potential for enhanced and well-controlled stem cell delivery for improved cardiac repair after a myocardial infarction. (C) 2014 Wiley Periodicals, Inc
收录类别	SCI
原文出处	http://onlinelibrary.wiley.com/doi/10.1002/bit.25337/abstract
语种	英语
内容类型	期刊论文
源URL	[http://ir.siat.ac.cn:8080/handle/172644/9161]
专题	深圳先进技术研究院_其他
作者单位	BIOTECHNOLOGY AND BIOENGINEERING
推荐引用方式 GB/T 7714	Kokhuis T. J. A.,Skachkov I.,Naaijkens B. A.,et al. Intravital Microscopy of Localized Stem Cell Delivery Using Microbubbles and Acoustic Radiation Force[J]. BIOTECHNOLOGY AND BIOENGINEERING,2015.
APA	Kokhuis T. J. A..,Skachkov I..,Naaijkens B. A..,Juffermans L. J. M..,Kamp O..,...&de Jong N.(2015).Intravital Microscopy of Localized Stem Cell Delivery Using Microbubbles and Acoustic Radiation Force.BIOTECHNOLOGY AND BIOENGINEERING.
MLA	Kokhuis T. J. A.,et al."Intravital Microscopy of Localized Stem Cell Delivery Using Microbubbles and Acoustic Radiation Force".BIOTECHNOLOGY AND BIOENGINEERING (2015).