The anti-inflammatory effect of Andrographis paniculata (Burm. f.) Nees on pelvic inflammatory disease in rats through down-regulation of the NF-κB pathway

	The anti-inflammatory effect of Andrographis paniculata (Burm. f.) Nees on pelvic inflammatory disease in rats through down-regulation of the NF-κB pathway
	Wei Zou; Zuoqi Xiao; Xiaoke Wen; Jieying Luo; Shuqiong Chen; Zeneng Cheng; Daxiong Xiang; Jian Hu; Jingyu He
刊名	BMC Complementary and Alternative Medicine
	2016
英文摘要	Background: Andrographis paniculata (Burm. f.) Nees (APN), a principal constituent of a famous traditional Chinese medicine Fukeqianjin tablet which is used for the treatment of pelvic inflammatory disease (PID), has been reported to have anti-inflammatory effect in vitro. However, whether it has pharmacological effect on PID in vivo is unclear. Therefore, the aim of this study is to test the anti-inflammatory effect of APN and illuminate a potential mechanism. Methods: Thirty-six female specific pathogen-free SD rats were randomly divided into control group, PID group, APN1 group, APN2 group, APN3 group and prednisone group. Pathogen-induced PID rats were constructed. The APN1, APN2 and APN3 group rats were orally administrated with APN extract at different levels. The prednisone group rats were administrated with prednisone. Eight days after the first infection, the histological examination of upper genital tract was carried out, and enzyme-linked immunosorbent assay (ELISA) was carried out using homogenate of the uterus and fallopian tube. Furthermore, immunohistochemical evaluations of NF-κB p65 and IκB-α in uterus was conducted. Results: APN obviously suppressed the infiltrations of neutrophils and lymphocytes, and it could significantly reduce the excessive production of cytokines and chemokines including IL-1β, IL-6, CXCL-1, MCP-1 and RANTES in a dose-dependent manner. Furthermore, APN could block the pathogen-induced activation of NF-κB pathway. Conclusion: APN showed potent anti-inflammatory effect on pathogen-induced PID in rats, with a potential mechanism of inhibiting the NF-κB signal pathway.
收录类别	SCI
原文出处	http://bmccomplementalternmed.biomedcentral.com/articles/10.1186/s12906-016-1466-5
语种	英语
内容类型	期刊论文
源URL	[http://ir.siat.ac.cn:8080/handle/172644/9761]
专题	深圳先进技术研究院_南沙所
作者单位	BMC Complementary and Alternative Medicine
推荐引用方式 GB/T 7714	Wei Zou,Zuoqi Xiao,Xiaoke Wen,et al. The anti-inflammatory effect of Andrographis paniculata (Burm. f.) Nees on pelvic inflammatory disease in rats through down-regulation of the NF-κB pathway[J]. BMC Complementary and Alternative Medicine,2016.
APA	Wei Zou.,Zuoqi Xiao.,Xiaoke Wen.,Jieying Luo.,Shuqiong Chen.,...&Jingyu He.(2016).The anti-inflammatory effect of Andrographis paniculata (Burm. f.) Nees on pelvic inflammatory disease in rats through down-regulation of the NF-κB pathway.BMC Complementary and Alternative Medicine.
MLA	Wei Zou,et al."The anti-inflammatory effect of Andrographis paniculata (Burm. f.) Nees on pelvic inflammatory disease in rats through down-regulation of the NF-κB pathway".BMC Complementary and Alternative Medicine (2016).