Cis-acting regulatory elements: from random screening to quantitative design

	Cis-acting regulatory elements: from random screening to quantitative design
	Hailin Meng; Yong Wang
刊名	Quantitative Biology
	2015
英文摘要	The cis-acting regulatory elements, e.g., promoters and ribosome binding sites (RBSs) with various desired properties, are building blocks widely used in synthetic biology for fine tuning gene expression. In the last decade, acquisition of a controllable regulatory element from a random library has been established and applied to control the protein expression and metabolic flux in different chassis cells. However, more rational strategies are still urgently needed to improve the efficiency and reduce the laborious screening and multifaceted characterizations. Building precise computational models that can predict the activity of regulatory elements and quantitatively design elements with desired strength have been demonstrated tremendous potentiality. Here, recent progress on construction of cisacting regulatory element library and the quantitative predicting models for design of such elements are reviewed and discussed in detail.
收录类别	其他
原文出处	http://link.springer.com/article/10.1007/s40484-015-0050-1
语种	英语
内容类型	期刊论文
源URL	[http://ir.siat.ac.cn:8080/handle/172644/7542]
专题	深圳先进技术研究院_南沙所
作者单位	Quantitative Biology
推荐引用方式 GB/T 7714	Hailin Meng,Yong Wang. Cis-acting regulatory elements: from random screening to quantitative design[J]. Quantitative Biology,2015.
APA	Hailin Meng,&Yong Wang.(2015).Cis-acting regulatory elements: from random screening to quantitative design.Quantitative Biology.
MLA	Hailin Meng,et al."Cis-acting regulatory elements: from random screening to quantitative design".Quantitative Biology (2015).