Genome-wide computational identification of microRNAs and their targets in the deep-branching eukaryote Giardia lamblia

CORC > 昆明动物研究所 > 昆明动物研究所 > 遗传资源与进化国家重点实验室 > 真核细胞进化基因组

	Genome-wide computational identification of microRNAs and their targets in the deep-branching eukaryote Giardia lamblia
	Zhang YQ 1,2; Chen DL 2; Tian HF 1; Zhang BH 3; Wen JF[*]1
刊名	COMPUTATIONAL BIOLOGY AND CHEMISTRY
	2009
卷号	33 期号:5 页码:391-396
关键词	MicroRNA Gene regulation Computational Giardia lamblia
ISSN号	1476-9271
通讯作者	wenjf@mail.kiz.ac.cn
合作状况	其它
英文摘要	Using a combined computational program. we identified 50 potential microRNAs (miRNAs) in Giardia lamblia. one of the most primitive unicellular eukaryotes. These miRNAs are unique to G. lamblia and no homologues have been found in other organisms; miRNAs. currently known in other species, were not found in G. lamblia. This suggests that miRNA biogenesis and miRNA-mediated gene regulation pathway may evolve independently. especially in evolutionarily distant lineages. A majority (43) of the predicted miRNAs are located at one single locus; however, some miRNAs have two or more copies in the genome. Among the 58 miRNA genes, 28 are located in the intergenic regions whereas 30 are present in the anti-sense strands of the protein-coding sequences. Five predicted miRNAs are expressed in G lamblia trophozoite cells evidenced by expressed sequence tags or RT-PCR. Thirty-seven identified miRNAs may target 50 protein-coding genes, including seven variant-specific surface proteins (VSPs). Our findings provide a clue that miRNA-mediated gene regulation may exist in the early stage of eukaryotic evolution, suggesting that it is an important regulation system ubiquitous in eukaryotes.
收录类别	SCI
资助信息	This work was supported by grants from the NSFC (30830018; 30021004; 30623007), 973 Program (2007CB815705), the Knowledge Innovation Program (KSCX2-YW-R-091), and grants (2006C0014Z; 2007C098M) from Yunnan province.
语种	英语
公开日期	2010-08-24
内容类型	期刊论文
源URL	[http://159.226.149.42:8088/handle/152453/5969]
专题	昆明动物研究所_真核细胞进化基因组昆明动物研究所_遗传资源与进化国家重点实验室
作者单位	1.State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, China 2.Graduate School of the Chinese Academy of Sciences, Beijing 100039, China 3.Department of Biology, East Carolina University, Greenville, NC 27858, United States
推荐引用方式 GB/T 7714	Zhang YQ,Chen DL,Tian HF,et al. Genome-wide computational identification of microRNAs and their targets in the deep-branching eukaryote Giardia lamblia[J]. COMPUTATIONAL BIOLOGY AND CHEMISTRY,2009,33(5):391-396.
APA	Zhang YQ,Chen DL,Tian HF,Zhang BH,&Wen JF[*].(2009).Genome-wide computational identification of microRNAs and their targets in the deep-branching eukaryote Giardia lamblia.COMPUTATIONAL BIOLOGY AND CHEMISTRY,33(5),391-396.
MLA	Zhang YQ,et al."Genome-wide computational identification of microRNAs and their targets in the deep-branching eukaryote Giardia lamblia".COMPUTATIONAL BIOLOGY AND CHEMISTRY 33.5(2009):391-396.