Opiate withdrawal induces dynamic-expressions of AMPA receptors and its regulatory molecule CaMKII alpha in hippocampal synapses

CORC > 昆明动物研究所 > 昆明动物研究所 > 动物模型与人类重大疾病机理重点实验室 > 学习记忆的分子神经机制

	Opiate withdrawal induces dynamic-expressions of AMPA receptors and its regulatory molecule CaMKII alpha in hippocampal synapses
	Zhong WX 1; Dong ZF 2; Xu L[]2,3,4; Luo JH[]1; Tian M 2; Cao J 2; Xu TL 3
刊名	LIFE SCIENCES
	2006
卷号	79 期号:9 页码:861-869
关键词	AMPA receptor phosphorylation CaMKII alpha opiate withdrawal synaptosome
通讯作者	lxu@vip.163.com ; luojianhong@zjuem.zju.edu.cn
英文摘要	Adaptive changes in brain areas following drug withdrawal are believed to contribute to drug seeking and relapse. Cocaine withdrawal alters the expression of GluR1 and GluR2/3 subunits of alpha-amino-3-hydroxy-5-methylisoxazole-4-propionic acid (AMPA) receptors in nucleus accumbens or amygdala, but the influence of drug withdrawal on hippocampus is little known. Here, we have examined the expression of GluR1 and GluR2/3 in hippocampal membrane and synaptic fractions following repeated morphine exposure and subsequent withdrawal. Repeated morphine exposure for 12 d increased GluR1 and GluR2/3 in synaptosome but not in membrane fraction. Interestingly, CaMKII alpha, known to be able to regulate the function of AMPA receptors, was decreased in synaptosome but not in membrane fraction; pCaMKII alpha, the phosphorylated form of CaMKII alpha, was increased in both fractions. However, during opiate withdrawal, GluR1 was generally reduced while GluR2/3 was prominently increased in both fractions; pCaMKII alpha was strongly decreased immediately after withdrawal, but detectably increased in late phase of morphine withdrawal in both fractions. Importantly, the opiate withdrawal-induced increase in GluR2/3 was dependent on the activation of glucocorticoid receptors and NMDA receptors, as it was prevented by the glucocorticoid receptor antagonist RU38486, or intrahippocampal injection of the NMDA receptor antagonist AP-5 or the antagonist to NR2B-containing NMDA receptors, Ro25-6981. These findings indicate that opiate withdrawal induces dynamic expression of GluR1 and GluR2/3 subunits of AMPA receptors in hippocampal synapses, possibly revealing an adaptive process of the hippocampal functions following opiate withdrawal.
收录类别	SCI
资助信息	This work was supported by grants from the National Science Foundation of China (30530250) and the National Basic Research Program (2006CB500800) to L.X. and J.H.L.
语种	英语
内容类型	期刊论文
源URL	[http://159.226.149.26:8080/handle/152453/10878]
专题	昆明动物研究所_学习记忆的分子神经机制
作者单位	1.Department of Neurobiology, Zhejiang University School of Medicine, Hangzhou 310006, PR China 2.Laboratory of Learning and Memory, Kunming Institute of Zoology, the Chinese Academy of Sciences, Kunming 650223, PR China 3.Institute of Neuroscience, Shanghai Institutes for Biological Sciences, the Chinese Academy of Sciences, Shanghai 200031, PR China 4.Mental Health Institute, the 2nd Hospital of Xiangya Medical College, Central South University, Changsha 410011, PR China
推荐引用方式 GB/T 7714	Zhong WX,Dong ZF,Xu L[*],et al. Opiate withdrawal induces dynamic-expressions of AMPA receptors and its regulatory molecule CaMKII alpha in hippocampal synapses[J]. LIFE SCIENCES,2006,79(9):861-869.
APA	Zhong WX.,Dong ZF.,Xu L[].,Luo JH[].,Tian M.,...&Xu TL.(2006).Opiate withdrawal induces dynamic-expressions of AMPA receptors and its regulatory molecule CaMKII alpha in hippocampal synapses.LIFE SCIENCES,79(9),861-869.
MLA	Zhong WX,et al."Opiate withdrawal induces dynamic-expressions of AMPA receptors and its regulatory molecule CaMKII alpha in hippocampal synapses".LIFE SCIENCES 79.9(2006):861-869.