异丙酚对大鼠海马CAl区神经元兴奋性突触传递的影响

CORC > 昆明动物研究所 > 昆明动物研究所 > 动物模型与人类重大疾病机理重点实验室 > 学习记忆的分子神经机制

	异丙酚对大鼠海马CAl区神经元兴奋性突触传递的影响
	谢玉波[*]1; 徐林 2; 熊文勇 2; 刘敬臣 1
刊名	中华麻醉学杂志
	2006
卷号	26 期号:5 页码:404-406
关键词	二异丙酚突触传递海马神经元
其他题名	Effects of propofoi Oil excitatory synaptic transmission in hippocampal CAl neurons in rats
通讯作者	xieyubo715001@yahoo.com.cn
合作状况	其它
中文摘要	目的研究异丙酚对大鼠海马CA1区神经元兴奋性突触后电流（EPSC）和自发性兴奋性突触后电流（sEPSC）的影响。方法 Wistar大鼠断头后分离海马脑组织，制成400μm厚度的海马脑片，脑片随机分为5组（n=10）。脂肪乳剂Ⅰ组、异丙酚Ⅰ组、SR95531＋异丙酚组：记录EPSC10min（基础值）后分别加入10％脂肪乳剂90μl,1％异丙酚90μl（相当于100μmol／L）、10μmol／LSR95531＋100μmol/L异丙酚，继续记录EPSC40min，分析EPSC幅值的变化。脂肪乳剂Ⅱ组、异丙酚Ⅱ组：细胞破膜后稳定10．15min，分别加入10％脂肪乳剂90出和1％异丙酚90出，记录sEPSC40min，分析sEPSC频率、幅值和半衰期的变化。膜钳制电压均为-70mV。结果与基础值比较，给药后脂肪乳剂Ⅰ组和SR95531＋异丙酚组EPSC幅值差异无统计学意义，异丙酚Ⅰ组EPSC幅值降低；给药后异丙酚Ⅰ组EPSC幅值比脂肪乳剂Ⅰ组降低（P〈0．05）。与脂肪乳剂Ⅱ组比较，异丙酚Ⅱ组sEPSC的频率、幅值降低、半衰期缩短（P〈0．05）。结论异丙酚主要通过增强大鼠海马CA1区神经元突触前膜和突触后膜的GABA．受体活性，产生突触前抑制和突触后抑制，从而抑制兴奋性突触传递。
英文摘要	Objective To investigate the effects of propofol on the whole-cell excitatory postsynaptic currents （EPSC） and spontaneous excitatory postsynaptic current （sEPSC） in hippocampal CA1 neurons. Methods Wistar rats （13-19 days old） weighing 40-60 g were decapitated and the hippocampi were immediately removed and placed in 0-4℃ artificial CSF aerated with 95% O2 and 5% CO2 The hippocampi were sliced （400 tan thick）. EPSCs were recorded in hippocampal CA1 neurons by stimulating the Schaffer collateral / commissural pathway. The 50 slices were divided into 5 groups （n = 10 each）： group Ⅰ intralipid-1; group Ⅱ propofol-1; group Ⅲ SR95531 ＋ propofol; group IV intralipid-2 and groupV propofol-2. In groupⅠ , Ⅱ and Ⅲ after EPSCs were recorded for 10 min, 10% intralipid 90μl, 1% propofol 90 μl （final concentration was 100 μmol·L^-1 and SR95531 10 μmol· L^-1 ＋ propofol 100 μmol·L^-1 were added to the perfusate and again EPSCs were recorded for 40 min. The changes in amplitude of EPSC were analyzed. In group IV and V after the cell membrane was perforated and being stabilized for 10-15 min 10% intralipid 90 μl and 1% propofol （100μmol·L^-1 ） was added to the perfusate and sEPSCs were recorded without stimulation. The holding potential was - 70 mV. Results Intralipid didn＇t affect EPSC but propfol 100μmol·L^-1 reduced EPSC to 47.7% of the baseline value. SR95531 could reverse the effect of propofol on EPSCs. The frequency, amplitude and decay time of sEPSC in group propofol-2 were reduced to 31.9% , 70.9% and 50.7% of those in group Ⅳ（intralipid-2）（P 〈 0.05）. Conclusion Propofol could inhibit excitatory transmission in hippocampal CA1 neurons by enhancing the activity of GABAA receptor on pre- and post-synaptic membrane.
收录类别	其他
语种	中文
公开日期	2010-08-09
内容类型	期刊论文
源URL	[http://159.226.149.42:8088/handle/152453/1078]
专题	昆明动物研究所_学习记忆的分子神经机制
作者单位	1.广西医科大学附属第一医院麻醉科,南宁市530021 2.中国科学院昆明动物研究所学习与记忆实验室
推荐引用方式 GB/T 7714	谢玉波[*],徐林,熊文勇,等. 异丙酚对大鼠海马CAl区神经元兴奋性突触传递的影响[J]. 中华麻醉学杂志,2006,26(5):404-406.
APA	谢玉波[*],徐林,熊文勇,&刘敬臣.(2006).异丙酚对大鼠海马CAl区神经元兴奋性突触传递的影响.中华麻醉学杂志,26(5),404-406.
MLA	谢玉波[*],et al."异丙酚对大鼠海马CAl区神经元兴奋性突触传递的影响".中华麻醉学杂志 26.5(2006):404-406.