Homologous feeder cells support undifferentiated growth and pluripotency in monkey embryonic stem cells

	Homologous feeder cells support undifferentiated growth and pluripotency in monkey embryonic stem cells
	Li TQ 1,2,3; Wang SF 1,2,3; Wang L 5; Yang SH 1,2; Wolf D 4; Zhou Q 5; Ji WZ[*]1,2,6; Xie YH 1,2,6; Lu YQ 1,2,3; Zhang XZ 1,2,3
刊名	STEM CELLS
	2005
卷号	23 期号:8 页码:1192-1199
关键词	embryonic stem cells rhesus monkey feeders stem cell markers self-renewal Wnt signaling
通讯作者	wji@mail.kiz.ac.cn
合作状况	其它
英文摘要	In the present study, five homologous feeder cell lines were developed for the culture and maintenance of rhesus monkey embryonic stem cells (rESCs). Monkey ear skin fibroblasts (MESFs), monkey oviductal fibroblasts (MOFs), monkey follicular granulosa fibroblast-like (MFG) cells, monkey follicular granulosa epithelium-like (MFGE) cells, and clonally derived fibroblasts from MESF (CMESFs) were established and compared with the ability of mouse embryonic fibroblasts (MEFs) to support rESC growth. MESF, MOF, MFG, and CMESF cells, but not MFGE cells, were as good as or better than MEFs in supporting undifferentiated growth while maintaining the differentiation potential of the rESCs. In an effort to understand the unique properties of supportive feeder cells, expression levels for a number of candidate genes were examined. MOF, MESF, and MEF cells highly expressed leukemia inhibitory factor, ciliary neurotrophic factor, basic fibroblast growth factor, stem cell factor, transforming growth factor PI, bone morphogenetic protein 4, and WNT3A, whereas WNT2, WNT4, and WNT5A were downregulated, compared with MFGE cells. Additionally, all monkey feeder cell lines expressed Dkk1 and LRP6, antagonists of the WNT signaling pathway, but not WNT1, WNT8B, or Dkk2. rESCs grown on homologous feeders maintained normal karyotypes, displayed the characteristics of ESCs, including morphology, alkaline phosphatase, Oct4, the cell surface markers stage-specific embryonic antigen (SSEA)-3, SSEA-4, tumor-related antigen (TRA)-1-60, and TRA-1-81, and formed cystic embryoid bodies in vitro that included differentiated cells representing the three major germ layers. These results indicate that the four homologous feeder cell lines can be used to support the undifferentiated growth and maintenance of pluripotency in rESCs.
收录类别	SCI
资助信息	This work was supported by research grants from Major State Research Development Program 2004CCA01300, G200016108 and 2001cb510100, The Chinese Academy of Sciences KSCX1- 05, Chinese National Science Foundation 30370166, and Yun- nan Nature Science Foundation 2001C0009Z. Tianqing Li and Shufen Wang contributed equally to this study.
语种	英语
内容类型	期刊论文
源URL	[http://159.226.149.26:8080/handle/152453/10603]
专题	昆明动物研究所_生殖与发育生物学昆明动物研究所_中国科学院昆明灵长类研究中心
作者单位	1.Kunming Primate Research Center, Chinese Academy of Sciences, Kunming, Yunnan, China 2.Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan, China 3.Graduate School, The Chinese Academy of Sciences, Beijing, China 4.Oregon National Primate Research Center, Portland, Oregon, USA 5.Institute of Zoology, Chinese Academy of Sciences, Beijing, China 6.Yunnan Key Laboratory for Animal Reproductive Biology, Kunming, Yunnan, China
推荐引用方式 GB/T 7714	Li TQ,Wang SF,Wang L,et al. Homologous feeder cells support undifferentiated growth and pluripotency in monkey embryonic stem cells[J]. STEM CELLS,2005,23(8):1192-1199.
APA	Li TQ.,Wang SF.,Wang L.,Yang SH.,Wolf D.,...&Zhang XZ.(2005).Homologous feeder cells support undifferentiated growth and pluripotency in monkey embryonic stem cells.STEM CELLS,23(8),1192-1199.
MLA	Li TQ,et al."Homologous feeder cells support undifferentiated growth and pluripotency in monkey embryonic stem cells".STEM CELLS 23.8(2005):1192-1199.