Generation and characterization of rabbit embryonic stem cells

	Generation and characterization of rabbit embryonic stem cells
	Wang SF 1,2,3; Tang XH 1,3; Li TQ 1,3; Zhang XZ 1,2,3; Hu ZX 1,2,3; Zhou Q 4; Ji WZ[*]1,3; Niu YY 1,2,3; Chen HW 1,2,3; Li B 1,2,3
刊名	STEM CELLS
	2007
卷号	25 期号:2 页码:481-489
关键词	rabbit embryonic stem cells parthenogenetic activation stem cell marker self-renewal signaling pathway
通讯作者	wji@mail.kiz.ac.cn
合作状况	其它
英文摘要	We described the derivation of four stable pluripotent rabbit embryonic stem cell ( ESC) lines, one ( RF) from blastocysts fertilized in vivo and cultured in vitro and three ( RP01, RP02, and RP03) from parthenogenetic blastocysts. These ESC lines have been cultivated for extended periods ( RF > 1 year, RP01 > 8 months, RP02 > 8 months, and RP03 > 6 months) in vitro while maintaining expression of pluripotent ESC markers and a normal XY or XX karyotype. The ESCs from all lines expressed alkaline phosphatase, transcription factor Oct- 4, stage- specific embryonic antigens ( SSEA-1, SSEA-3, and SSEA-4), and the tumor- related antigens ( TRA-1-60 and TRA-1-81). Similar to human and mouse ESCs, rabbit ESCs expressed pluripotency ( Oct- 4, Nanog, SOX2, and UTF-1) and signaling pathway genes ( fibroblast growth factor, WNT, and transforming growth factor pathway). Morphologically, rabbit ESCs resembled primate ESCs, whereas their proliferation characteristics were more like those seen in mouse ESCs. Rabbit ESCs were induced to differentiate into many cell types in vitro and formed teratomas with derivatives of the three major germ layers in vivo when injected into severe combined immunodeficient mice. Our results showed that pluripotent, stable ESC lines could be derived from fertilized and parthenote-derived rabbit embryos.
收录类别	SCI
资助信息	This work was supported by research grants from Major State Re- search Development Program 2004CCA01300 and 2006CB701500, The Chinese Academy of Sciences KSCX1-05, KSCX1-YW- R-47, Chinese National Science Foundation 30370166 and 30570906.
语种	英语
内容类型	期刊论文
源URL	[http://159.226.149.26:8080/handle/152453/10542]
专题	昆明动物研究所_生殖与发育生物学昆明动物研究所_中国科学院昆明灵长类研究中心
作者单位	1.Kunming Primate Research Center, and Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming Yunnan, China 2.Graduate School, Chinese Academy of Sciences, Beijing, China 3.Yunnan Key Laboratory of Animal Reproductive Biology, Kunming Yunnan, China 4.Institute of Zoology, Chinese Academy of Sciences, Beijing, China
推荐引用方式 GB/T 7714	Wang SF,Tang XH,Li TQ,et al. Generation and characterization of rabbit embryonic stem cells[J]. STEM CELLS,2007,25(2):481-489.
APA	Wang SF.,Tang XH.,Li TQ.,Zhang XZ.,Hu ZX.,...&Li B.(2007).Generation and characterization of rabbit embryonic stem cells.STEM CELLS,25(2),481-489.
MLA	Wang SF,et al."Generation and characterization of rabbit embryonic stem cells".STEM CELLS 25.2(2007):481-489.