Comparative analysis of macaque and human sperm proteomes: Insights into sperm competition

CORC > 昆明动物研究所 > 昆明动物研究所 > 中国科学院昆明灵长类研究中心

	Comparative analysis of macaque and human sperm proteomes: Insights into sperm competition
	Zhou T 1; Wang GG 1; Yu CM 1; Zhou ZM 1,2; Si W 3; Sha JH 1; Guo XJ[*]1; Chen MJ 2; Zhang MQ 1; Guo YS 1
刊名	PROTEOMICS
	2015
卷号	15 期号:9 页码:1564-1573
关键词	Animal proteomics Positive selection Rhesus macaque Sperm competition Sperm motility Sperm proteome
通讯作者	guo_xuejiang@njmu.edu.cn
英文摘要	Male macaques produce faster sperm than male humans due to a higher pressure of sperm competition in macaques. To explore the molecular basis of this biological difference, we firstly constructed macaque and human sperm proteomes using LC-MS/MS. We then detected the positively selected genes specifically on the branch of macaque based on branch-site likelihood method. We identified 197 positively selected genes specifically on the branch of macaque that are unselected in corresponding human orthologs. These genes are highly associated with mitochondria and axoneme that directly drive sperm motility. We further compared the ultrastructural differences of the midpiece between macaque and human sperms to provide evidence for our findings using transmission electron microscopy. In conclusion, our results provide potential molecular targets for explaining the different phenotypes under sperm competition between macaques and humans, and also provide resources for the analysis of male fertility.
收录类别	SCI
资助信息	This study was supported by grants from Chinese Natural Science Funds (81222006), the 973 programs (2011CB944304 and 2013CB911400), and the Chinese Natural Science Fund (31271245). It was also sponsored by Natural Science Founda- tionoftheJiangsuHigherEducationInstitutionsofChina(Grant No. 13KJA310002), and was supported by Grant SKLRM-KF- 1201.
语种	英语
内容类型	期刊论文
源URL	[http://159.226.149.26:8080/handle/152453/10339]
专题	昆明动物研究所_中国科学院昆明灵长类研究中心昆明动物研究所_生殖与发育生物学
作者单位	1.State Key Laboratory of Reproductive Medicine, Department of Histology and Embryology, Nanjing Medical University, Nanjing, P . R. China 2.Key Laboratory of Modern Toxicology of Ministry of Education, School of Public Health, Nanjing Medical University, Nanjing, P . R. China 3.Kunming Primate Research Centre and Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, P . R. China
推荐引用方式 GB/T 7714	Zhou T,Wang GG,Yu CM,et al. Comparative analysis of macaque and human sperm proteomes: Insights into sperm competition[J]. PROTEOMICS,2015,15(9):1564-1573.
APA	Zhou T.,Wang GG.,Yu CM.,Zhou ZM.,Si W.,...&Guo YS.(2015).Comparative analysis of macaque and human sperm proteomes: Insights into sperm competition.PROTEOMICS,15(9),1564-1573.
MLA	Zhou T,et al."Comparative analysis of macaque and human sperm proteomes: Insights into sperm competition".PROTEOMICS 15.9(2015):1564-1573.