Efficient generation of biliary epithelial cells from rabbit intrahepatic bile duct by Y-27632 and Matrigel

CORC > 昆明动物研究所 > 昆明动物研究所 > 中国科学院昆明灵长类研究中心

	Efficient generation of biliary epithelial cells from rabbit intrahepatic bile duct by Y-27632 and Matrigel
	Jin LF 1; Sun AJ[*]2; Ji SH 1,3
刊名	IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL
	2013
卷号	49 期号:6 页码:433-439
关键词	Rabbit Biliary epithelial cells Culture Y-27632 Matrigel
通讯作者	sun_aijing@hotmail.co.jp
合作状况	其它
英文摘要	Efficient culture of primary biliary epithelial cells (BECs) from adult liver is useful for both experimental studies and clinical applications of tissue engineering. However, an effective culture system for long-term proliferation of adult BECs is still unachieved. Laboratory rabbit has been used in a large number of studies; however, there are no reports of BECs from normal adult rabbit. As little as 5 g of normal rabbit liver tissue were minced, digested, and then clonally cultured in medium containing FBS and ITS. Cells were characterized by cell morphology, immunoassaying, and growth rate assay. Different combination of growth factors and substrates, including Y-27632 and Matrigel, were employed to assess their effect on cell proliferation. In the primary culture, the BECs cellular sheets consisting of cuboidal cells, as well as fibroblast-like cells and other hepatic cells, emerged with time of culture. The BECs cellular sheets were then manually split into cells clumps for further characterization. The subcultured cells had typical cell morphology of cholangiocytes, expressed the specific markers of BECs, including GGT, cytokeratin (CK18), and CK19, and possessed the capacity to form duct-like structure in three-dimensional Matrigel. Y-27632 and Matrigel-treated BECs had a steady growth rate as well as colony-formation capacity. The BECs were maintained in Y-27632 and Matrigel culture system for more than 3 mo. This is the first example, to our knowledge, of the successful culture of BECs from normal adult rabbit liver. Furthermore, our results indicate that treatment of BECs with Y-27632 and Matrigel is a simple method for efficient output of BECs.
收录类别	SCI
资助信息	This work was supported by research grants from Zhejiang Provincial Natural Science Foundation of China Y2110911 and Zhejiang Provincial Key Medical Discipline Construc- tion Program of China GJSX-0100-004.
语种	英语
WOS记录号	WOS:000320279500006
公开日期	2013-07-12
内容类型	期刊论文
源URL	[http://159.226.149.42:8088/handle/152453/7567]
专题	昆明动物研究所_中国科学院昆明灵长类研究中心昆明动物研究所_生殖与发育生物学
作者单位	1.College of Life Science, Shaoxing University, Shaoxing, Zhejiang, China 2.Department of Pathology, Shaoxing People’s Hospital, Shaoxing hospital of Zhejiang University, Shaoxing, Zhejiang, China 312000 3.Kunming Primate Research Center and Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan, China
推荐引用方式 GB/T 7714	Jin LF,Sun AJ[*],Ji SH. Efficient generation of biliary epithelial cells from rabbit intrahepatic bile duct by Y-27632 and Matrigel[J]. IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL,2013,49(6):433-439.
APA	Jin LF,Sun AJ[*],&Ji SH.(2013).Efficient generation of biliary epithelial cells from rabbit intrahepatic bile duct by Y-27632 and Matrigel.IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL,49(6),433-439.
MLA	Jin LF,et al."Efficient generation of biliary epithelial cells from rabbit intrahepatic bile duct by Y-27632 and Matrigel".IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL 49.6(2013):433-439.