The Positive Effects of Seminal Plasma During the Freezing Process on Cryosurvival of Sperm with Poor Freezability in the Rhesus Macaque (Macaca mulatta)

	The Positive Effects of Seminal Plasma During the Freezing Process on Cryosurvival of Sperm with Poor Freezability in the Rhesus Macaque (Macaca mulatta)
	Yang SH 1,2,3; Ping SH 1,2,3; Ji SH 1,2,4; Lu YQ 1,2; Niu YY 1,2,4; Wang H 1,2,4; Ji WZ 1,2,4; Si W[*]1,2,4
刊名	JOURNAL OF REPRODUCTION AND DEVELOPMENT
	2011
卷号	57 期号:6 页码:737-743
关键词	Cryopreservation Rhesus macaque Seminal plasma Sperm
通讯作者	siw@mail.kiz.ac.cn
合作状况	其它
英文摘要	The objective was to examine the effect of seminal plasma on cryopreservation of sperm from rhesus macaques. Sperm cryosurvival was evaluated by sperm motility and acrosomal integrity. Compared with slow cooling (-0.4 C/min) from 37 C (body temperature) to 4 C, rapid cooling (-16 C/min) caused cold shock in rhesus macaque sperm. The cryosurvival of sperm was decreased regardless of the presence or absence of seminal plasma (P<0.05). However, the presence of seminal plasma during cold shock at a rapid cooling rate improved sperm motility and acrosoma I integrity in individual monkeys. Male-to-male variation in sperm cryosurvival was observed after cryopreservation (P<0.05), and the presence of seminal plasma during sperm cryopreservation improved sperm motility and acrosomal integrity in individual monkeys (P<0.05). Furthermore, by adding seminal plasma from monkeys with good sperm cryosurvival to sperm freezing extender, the frozen-thawed motility and acrosomal integrity of sperm from monkey with poor cryosurvival were improved (P<0.05). The present study indicated that seminal fluid is beneficial to sperm undergoing cold shock or cryopreservation in individual monkeys. The cryosurvival of sperm from rhesus macaques with poor sperm freezability could be improved by the presence of seminal plasma from males with good sperm cryosurvival. This finding provides a useful method for genetic preservation in this important species
收录类别	SCI
语种	英语
公开日期	2012-03-05
内容类型	期刊论文
源URL	[http://159.226.149.42:8088/handle/353002/6856]
专题	昆明动物研究所_生殖与发育生物学
作者单位	1.Kunming Univ Sci & Technol, Lab Mol Genet Aging & Tumor, Fac Life Sci & Technol, Kunming 650224, Yunnan, Peoples R China 2.Chinese Acad Sci, Kunming Inst Zool, Yunnan Key Lab Anim Reprod, Kunming 650223, Yunnan, Peoples R China 3.Kunming Univ Sci & Technol, Lab Mol Genet Aging & Tumor, Fac Life Sci & Technol, Kunming 650224, Yunnan, Peoples R China 4.Natl Engn Res Ctr Biomed & Anim Sci, Kunming Biomed Int, Kunming 650500, Yunnan, Peoples R China
推荐引用方式 GB/T 7714	Yang SH,Ping SH,Ji SH,et al. The Positive Effects of Seminal Plasma During the Freezing Process on Cryosurvival of Sperm with Poor Freezability in the Rhesus Macaque (Macaca mulatta)[J]. JOURNAL OF REPRODUCTION AND DEVELOPMENT,2011,57(6):737-743.
APA	Yang SH.,Ping SH.,Ji SH.,Lu YQ.,Niu YY.,...&Si W[*].(2011).The Positive Effects of Seminal Plasma During the Freezing Process on Cryosurvival of Sperm with Poor Freezability in the Rhesus Macaque (Macaca mulatta).JOURNAL OF REPRODUCTION AND DEVELOPMENT,57(6),737-743.
MLA	Yang SH,et al."The Positive Effects of Seminal Plasma During the Freezing Process on Cryosurvival of Sperm with Poor Freezability in the Rhesus Macaque (Macaca mulatta)".JOURNAL OF REPRODUCTION AND DEVELOPMENT 57.6(2011):737-743.