Developmental potential of vitrified holstein cattle embryos fertilized in vitro with sex-sorted sperm

	Developmental potential of vitrified holstein cattle embryos fertilized in vitro with sex-sorted sperm
	Xu J 1; Guo Z 2; Su L 3; Nedambale TL 1; Zhang J 4; Schenk J 5; Moreno JF 6; Dinnyes A 7; Ji W 2; Tian XC 4
刊名	JOURNAL OF DAIRY SCIENCE
	2006
卷号	89 期号:7 页码:2510-2518
关键词	sorted sperm in vitro fertilization vitrification embryo transfer
ISSN号	0022-0302
通讯作者	fuliangd@evergen.com
合作状况	其它
英文摘要	In vitro fertilization (IVF) is a feasible way to utilize sex-sorted sperm to produce offspring of a predetermined sex in the livestock industry. The objective of the present study was to examine the effects of various factors on bovine IVF and to systematically improve the efficiency of IVF production using sex-sorted sperm. Both bulls and sorting contributed to the variability among differential development rates of embryos fertilized by sexed sperm. Increased sorting pressures (275.8 to 344.75 kPa) did not have a significant effect on the in vitro fertility of the sorted sperm; neither did an extended period of 9 to 14 h from semen collection to sorting. As few as 600 sorted sperm were used to fertilize an oocyte, resulting in blastocyst development of 33.2%. Postwarming of vitrified sexed IVF embryos resulted in high morphological survival (96.3%) and hatching (84.4%) rates, similar to those fertilized by nonsexed sperm (93.1 and 80.6%, respectively). A 40.9% pregnancy rate was established following the transfer of 3,627 vitrified, sexed embryos into synchronized recipients. This was not different from the rates with nonsexed IVF (41.9%, n = 481), or in vivo-produced (53.1%, n = 192) embryos. Of 458 calves born, 442 (96.5%) were female and 99.6% appeared normal. These technologies (sperm sexing-IVF-vitrification-embryo transfer) provide farmers, as well as the livestock industry, with a valuable option for herd expansion and heifer replacement programs. In summary, calves were produced using embryos fertilized by sex-sorted sperm in vitro and cryopreserved by rapid cooling vitrification.
收录类别	SCI
资助信息	This study was supported by the Small Business Innovation Research program of the USDA Cooperative State Research, Education, and Ex- tension Service (CSREES), Grant Number 2005-00178 to Fuliang Du, and Yankee Ingenuity Competition from Connecticut Innovation, Inc., to Xiangzhong Yang.
原文出处	2006892510.pdf
语种	英语
公开日期	2010-08-24
内容类型	期刊论文
源URL	[http://159.226.149.42:8088/handle/152453/5599]
专题	昆明动物研究所_生殖与发育生物学
作者单位	1.Evergen Biotechnologies, Inc., Storrs, CT 06269 2.Xinjiang Academy of Animal Science, the Key Laboratory of Animal Biotechnology of Ministry of Agriculture, Urumqi, Xinjiang 830000, China 3.Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, China 4.Department of Animal Science/Center for Regenerative Biology, University of Connecticut, Storrs 06269 5.XY Inc., Fort Collins, CO 80524 6.Sexing Technologies, Navasota, TX 77868 7.Department of Animal Biology, Agricultural Biotechnology Center, Godollo, Hungary
推荐引用方式 GB/T 7714	Xu J,Guo Z,Su L,et al. Developmental potential of vitrified holstein cattle embryos fertilized in vitro with sex-sorted sperm[J]. JOURNAL OF DAIRY SCIENCE,2006,89(7):2510-2518.
APA	Xu J.,Guo Z.,Su L.,Nedambale TL.,Zhang J.,...&Du F[*].(2006).Developmental potential of vitrified holstein cattle embryos fertilized in vitro with sex-sorted sperm.JOURNAL OF DAIRY SCIENCE,89(7),2510-2518.
MLA	Xu J,et al."Developmental potential of vitrified holstein cattle embryos fertilized in vitro with sex-sorted sperm".JOURNAL OF DAIRY SCIENCE 89.7(2006):2510-2518.