Regulation of zygotic gene activation in the preimplantation mouse embryo: Global activation and repression of gene expression

	Regulation of zygotic gene activation in the preimplantation mouse embryo: Global activation and repression of gene expression
	Ma J 3,4; Svoboda P 3; Schultz RM[*]2,3; Stein P 3
刊名	BIOLOGY OF REPRODUCTION
	2001
卷号	64 期号:6 页码:1713-1721
关键词	developmental biology early development embryo gene regulation
ISSN号	0006-3363
通讯作者	rschultz@mail.sas.upenn.edu
合作状况	其它
英文摘要	Superimposed on the activation of the embryonic genome in the preimplantation mouse embryo is the formation of a transcriptionally repressive state during the two-cell stage. This repression appears mediated at the level of chromatin structure, because it is reversed by inducing histone hyperacetylation or inhibiting the second round of DNA replication. We report that of more than 200 amplicons analyzed by mRNA differential display, about 45% of them are repressed between the two-cell and four-cell stages. This repression is scored as either a decrease in amplicon expression that occurs between the two-cell and four-cell stages or on the ability of either trichostatin A tan inhibitor of histone deacetylases) or aphidicolin tan inhibitor of replicative DNA polymerases) to increase the level of amplicon expression. Results of this study also indicate that about 16% of the amplicons analyzed likely are novel genes whose sequence doesn't correspond to sequences in the current databases, whereas about 20% of the sequences expressed during this transition likely are repetitive sequences. Lastly, inducing histone hyperacetylation in the two-cell embryos inhibits cleavage to the four-cell stage. These results suggest that genome activation is global and relatively promiscuous and that a function of the transcriptionally repressive state is to dictate the appropriate profile of gene expression that is compatible with further development.
收录类别	SCI
资助信息	This research was supported by grant HD 22681 from the National In- stitutes of Health to R.M.S. J.M. was supported by the Major State Basic Research Development Program (G2000016108) of the Chinese Academy of Sciences to the Kunming Institute of Zoology, Kunming, Yunnan Prov- ince, China.
原文出处	2001641713.pdf
语种	英语
公开日期	2010-08-24
内容类型	期刊论文
源URL	[http://159.226.149.42:8088/handle/152453/5227]
专题	昆明动物研究所_生殖与发育生物学
作者单位	1. 2.* 3.Department of Biology,University of Pennsylvania, Philadelphia, Pennsylvania 19104-6018 4.Kunming Institute of Zoology,Kunming, Yunnan Province, China
推荐引用方式 GB/T 7714	Ma J,Svoboda P,Schultz RM[*],et al. Regulation of zygotic gene activation in the preimplantation mouse embryo: Global activation and repression of gene expression[J]. BIOLOGY OF REPRODUCTION,2001,64(6):1713-1721.
APA	Ma J,Svoboda P,Schultz RM[*],&Stein P.(2001).Regulation of zygotic gene activation in the preimplantation mouse embryo: Global activation and repression of gene expression.BIOLOGY OF REPRODUCTION,64(6),1713-1721.
MLA	Ma J,et al."Regulation of zygotic gene activation in the preimplantation mouse embryo: Global activation and repression of gene expression".BIOLOGY OF REPRODUCTION 64.6(2001):1713-1721.