DNA barcoding and the identification of tree frogs (Amphibia: Anura: Rhacophoridae)

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	DNA barcoding and the identification of tree frogs (Amphibia: Anura: Rhacophoridae)
	Dang NX 1,2; Sun FH 3; Murphy RW 5; Wang WZ[]6; Li JT[]1; Lv YY 1,2; Zhao BH 1,2; Wang JC 4
刊名	MITOCHONDRIAL DNA
	2016
卷号	27 期号:4 页码:2574-2584
关键词	Cytochrome c oxidase subunit I gene cryptic species molecular identification rhacophorid
通讯作者	wangwz@mail.kiz.ac.cn ; lijt@cib.ac.cn
英文摘要	he DNA barcoding gene COI (cytochrome c oxidase subunit I) effectively identifies many species. Herein, we barcoded 172 individuals from 37 species belonging to nine genera in Rhacophoridae to test if the gene serves equally well to identify species of tree frogs. Phenetic neighbor joining and phylogenetic Bayesian inference were used to construct phylogenetic trees, which resolved all nine genera as monophyletic taxa except for Rhacophorus, two new matrilines for Liuixalus, and Polypedates leucomystax species complex. Intraspecific genetic distances ranged from 0.000 to 0.119 and interspecific genetic distances ranged from 0.015 to 0.334. Within Rhacophorus and Kurixalus, the intra- and interspecific genetic distances did not reveal an obvious barcode gap. Notwithstanding, we found that COI sequences unambiguously identified rhacophorid species and helped to discover likely new cryptic species via the synthesis of genealogical relationships and divergence patterns. Our results supported that COI is an effective DNA barcoding marker for Rhacophoridae.
收录类别	SCI
资助信息	This study was supported by the National Natural Science Foundation of China (31372173, 31411130190, 31101618), foundation of Ministry of Education Key Laboratory for Tropical Animal and Plant Ecology, Hainan Normal University (No. 201501 to J.T. Li), and grants from the Ministry of Science and Technology of China (MOST Grant 2011FY120200, Grant 2005DKA21402).
语种	英语
内容类型	期刊论文
源URL	[http://159.226.149.26:8080/handle/152453/10326]
专题	昆明动物研究所_生命条形码中心昆明动物研究所_遗传资源与进化国家重点实验室
作者单位	1.Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, China 2.College of Life Sciences, Sichuan University, Chengdu, China 3.School of Medical Laboratory Science, Chengdu Medical College, Chengdu, China 4.Department of Tropical Animal and Plant Ecology, College of Life Sciences, Hainan Normal University, Haikou, China 5.Department of Conservation Biology, Royal Ontario Museum, Toronto, Ontario, Canada 6.State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, China
推荐引用方式 GB/T 7714	Dang NX,Sun FH,Murphy RW,et al. DNA barcoding and the identification of tree frogs (Amphibia: Anura: Rhacophoridae)[J]. MITOCHONDRIAL DNA,2016,27(4):2574-2584.
APA	Dang NX.,Sun FH.,Murphy RW.,Wang WZ[].,Li JT[].,...&Wang JC.(2016).DNA barcoding and the identification of tree frogs (Amphibia: Anura: Rhacophoridae).MITOCHONDRIAL DNA,27(4),2574-2584.
MLA	Dang NX,et al."DNA barcoding and the identification of tree frogs (Amphibia: Anura: Rhacophoridae)".MITOCHONDRIAL DNA 27.4(2016):2574-2584.