A Robust Single Primate Neuroepithelial Cell Clonal Expansion System for Neural Tube Development and Disease Studies

CORC > 昆明动物研究所 > 昆明动物研究所 > 动物模型与人类重大疾病机理重点实验室 > 神经系统编码

	A Robust Single Primate Neuroepithelial Cell Clonal Expansion System for Neural Tube Development and Disease Studies
	Zhu XQ 1,3; Li B 1,2; Ai ZY 1,3; Xiang Z 1,2; Zhang KS 5; Qiu XY 1; Chen YC 1,3; Li YM 2; Rizak JD 4; Niu YY 1,3
刊名	STEM CELL REPORTS
	2016
卷号	6 期号:2 页码:228-242
通讯作者	wji@kbimed.com , litq@kbimed.com
合作状况	其它
英文摘要	Developing a model of primate neural tube (NT) development is important to promote many NT disorder studies in model organisms. Here, we report a robust and stable system to allow for clonal expansion of single monkey neuroepithelial stem cells (NESCs) to develop into miniature NT-like structures. Single NESCs can produce functional neurons in vitro, survive, and extensively regenerate neuron axons in monkey brain. NT formation and NESC maintenance depend on high metabolism activity and Wnt signaling. NESCs are regionally restricted to a telencephalic fate. Moreover, single NESCs can turn into radial glial progenitors (RGPCs). The transition is accurately regulated by Wnt signaling through regulation of Notch signaling and adhesion molecules. Finally, using the "NESC-TO-NTs'' system, we model the functions of folic acid (FA) on NT closure and demonstrate that FA can regulate multiple mechanisms to prevent NT defects. Our system is ideal for studying NT development and diseases.
收录类别	SCI
资助信息	This work was funded by grants from the Chinese Ministry of Science and Technology 973 program (2012CBA01307), the Key Technologies Research and Development Program of China (2014BAI03B01), the National Natural Science Foundation of China (31360231 and 31271599), and Yunnan Basic Research Pro- jects (2014FC004).
语种	英语
内容类型	期刊论文
源URL	[http://159.226.149.26:8080/handle/152453/9484]
专题	昆明动物研究所_神经系统编码
作者单位	1.Yunnan Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, 650500 Yunnan, China 2.Chongqing Key Lab of Forage & Herbivore, College of Animal Science and Technology (CAST), Southwest University, No. 1 Tiansheng Road, Beibei, Chongqing 400715, China 3.National Engineering Research Center of Biomedicine and Animal Science, Kunming 650500, China 4.Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, 650223 Yunnan, China 5.Translational Stem Cell Research Center, Tongji Hospital, Tongji University School of Medicine, Shanghai 200092, China
推荐引用方式 GB/T 7714	Zhu XQ,Li B,Ai ZY,et al. A Robust Single Primate Neuroepithelial Cell Clonal Expansion System for Neural Tube Development and Disease Studies[J]. STEM CELL REPORTS,2016,6(2):228-242.
APA	Zhu XQ.,Li B.,Ai ZY.,Xiang Z.,Zhang KS.,...&Li TQ[*].(2016).A Robust Single Primate Neuroepithelial Cell Clonal Expansion System for Neural Tube Development and Disease Studies.STEM CELL REPORTS,6(2),228-242.
MLA	Zhu XQ,et al."A Robust Single Primate Neuroepithelial Cell Clonal Expansion System for Neural Tube Development and Disease Studies".STEM CELL REPORTS 6.2(2016):228-242.