Discovery of differentially expressed genes in cashmere goat (Capra hircus) hair follicles by RNA sequencing

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	Discovery of differentially expressed genes in cashmere goat (Capra hircus) hair follicles by RNA sequencing
	Qiao X 1; Wu JH 2,3,4; Zhang YJ 1; Wang RJ 1; Zhao YH 1; Pan YX 1; Zhang WG[]1; Li JQ 1; Wu RB 5; Su R[]1,4
刊名	GENETICS AND MOLECULAR RESEARCH
	2016
卷号	15 期号:3 页码:gmr.15038589
关键词	Cashmere goat Differentially expressed gene Hair follicle RNA-Seq Whole transcriptome sequencing
通讯作者	szhang_sau@126.com ; yihsuanp@gmail.com
合作状况	其它
英文摘要	The mammalian hair follicle (HF) is a unique, highly regenerative organ with a distinct developmental cycle. Cashmere goat (Capra hircus) HFs can be divided into two categories based on structure and development time: primary and secondary follicles. To identify differentially expressed genes (DEGs) in the primary and secondary HFs of cashmere goats, the RNA sequencing of six individuals from Arbas, Inner Mongolia, was performed. A total of 617 DEGs were identified; 297 were upregulated while 320 were downregulated. Gene ontology analysis revealed that the main functions of the upregulated genes were electron transport, respiratory electron transport, mitochondrial electron transport, and gene expression. The downregulated genes were mainly involved in cell autophagy, protein complexes, neutrophil aggregation, and bacterial fungal defense reactions. According to the Kyoto Encyclopedia of Genes and Genomes database, these genes are mainly involved in the metabolism of cysteine and methionine, RNA polymerization, and the MAPK signaling pathway, and were enriched in primary follicles. A microRNA-target network revealed that secondary follicles are involved in several important biological processes, such as the synthesis of keratin-associated proteins and enzymes involved in amino acid biosynthesis. In summary, these findings will increase our understanding of the complex molecular mechanisms of HF development and cycling, and provide a basis for the further study of the genes and functions of HF development.
收录类别	其他
资助信息	Research supported by the National “863” Project (#2013AA102506), the National Natural Science Foundation of China (#31201773, #31260539, #31272421, and #31402052), the Excellent Young Scientist Foundation of Inner Mongolia Agricultural University of China (#2014XYQ-1), the Natural Science Foundation of Inner Mongolia (#2013MS0414), the Youth Innovation Foundation of Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences (#2013QNJJM03), and the State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences (#GREKF13-02).
语种	英语
内容类型	期刊论文
源URL	[http://159.226.149.26:8080/handle/152453/10524]
专题	昆明动物研究所_遗传资源与进化国家重点实验室
作者单位	1.College of Animal Science, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, China 2.Inner Mongolia Prataculture Research Center, Chinese Academy of Science, Hohhot, China 3.Animal Husbandry Institute, Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences, Hohhot, China 4.State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, China 5.College of Basic Medical Sciences, Inner Mongolia Medical University, Hohhot, China 6.College of Animal Science and Technology, Inner Mongolia University for Nationalities, Tongliao, Inner Mongolia, China
推荐引用方式 GB/T 7714	Qiao X,Wu JH,Zhang YJ,et al. Discovery of differentially expressed genes in cashmere goat (Capra hircus) hair follicles by RNA sequencing[J]. GENETICS AND MOLECULAR RESEARCH,2016,15(3):gmr.15038589.
APA	Qiao X.,Wu JH.,Zhang YJ.,Wang RJ.,Zhao YH.,...&Li C.(2016).Discovery of differentially expressed genes in cashmere goat (Capra hircus) hair follicles by RNA sequencing.GENETICS AND MOLECULAR RESEARCH,15(3),gmr.15038589.
MLA	Qiao X,et al."Discovery of differentially expressed genes in cashmere goat (Capra hircus) hair follicles by RNA sequencing".GENETICS AND MOLECULAR RESEARCH 15.3(2016):gmr.15038589.