An allozyme electrophoresis study on eleven species of megophryinae in China

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	An allozyme electrophoresis study on eleven species of megophryinae in China
	Liu WZ; Yang DT
刊名	动物学研究
	1994
卷号	15 期号:s1 页码:182-192
关键词	Amphibia Pelobatidae Megophryinae Genetic diversity Phylogenetic relationships
其他题名	中国角蟾亚科十一种的同工酶电泳研究
合作状况	其它
中文摘要	本文用同工酶电泳的方法分析了中国产角蟾亚科3属11种,共13个地理种群样本,以探讨它们的遗传多样性及系统关系。用于分析的酶共14种,由24个基因位点编码。经过酶带的分析和计算,得出了每个种群样本在每个基因位点上的等位基因频率（见表3）。根据基因频率的分布,计算得出了各种群样本的基因多样性指数——平均杂合率（H）（见表4）,结果表明,角蟾亚科的H值平均值为0.18, 在两栖动物中是属于很高的遗传多样性水平,根据基因频率的分布,计算了所有种群样本间的Nei’s遗传距（见表5）,并通过类平均法（UPGMA）重建了进化树（dendrogram）。分析结果支持将拟角蟾属（Ophryophryne）从角蟾属（Megophrys）中分出为独立属的观点;但是对短腿蟾属（Brachytarsophrys）是否为独立属提出了疑问。在角蟾属（Mesophrys）中,可以明显地看出3个组,它们中的种间亲缘关系比较近,这3个组分别是:①白颌大角蟾（M.lateralis）、大花角蟾（M.giganticus）和长肢角蟾（M.longipes）;②粗皮角蟾（M.palpebralespinneosa）、淡肩角蟾（M.boettgeri）和凹顶角蟾（M.parva）;③小角蟾（M.minor）和褂墩角蟾（M.kuatunensis）。峨嵋角蟾（M.omeimontis）3个地理种群聚为一支,互相间有一定的遗传差异,这与该种地理种群间的形态差异相.
英文摘要	Allozymes of eleven species of Megophryinae in China were exmined electrophoretically to investigate genetic diversity and phylogenetic relationships. Fourteen enzymes, presumptively coded by 24 lovi were detected to be variable.Gene frequencies of each population at each locus were presented. The commonly used measure of genetic diversity, the average heterozygosity (H) were calculated based on gene frequencies. The results indicated that Megophryinae had a high level of genetic diversity in amphibians, an average H of 0.18, ranging from 0.058 to 0.28. Nei's (1978)genetic distances (Nei's D) were calculated for all possible population pairs. A dedrogram of 13 populations represnenting 11 species, 3 genera of Megophryinae were derived and presented by using UPGMA, based on Nei's D. The assignment of O phryophryne as a distinct genus were supported by an average Nei's D of 1.4067 which separated O. microstoma from all other populations. Subdivision of Brachytarsophrys from Megophrys was not supported by this study. Within Megophrys, three groups were recognized: 1. m. lateralis, M. giganticus and M. longipes; 2. M palpebralespineosa, M. boettgeri and M. parva; 3. M. minor and M. kuatunensis. Three populations of M. omeimontis were closely related and share a clade independent form all other Megophrys, and B feae as well
收录类别	其他
资助信息	This work was financially supported by Natural Science Foundation of China
语种	英语
公开日期	2010-08-11
内容类型	期刊论文
源URL	[http://159.226.149.42:8088/handle/152453/1333]
专题	昆明动物研究所_其他
作者单位	Kunming Insititute of Zoology, The chinese Academy of Scienses
推荐引用方式 GB/T 7714	Liu WZ,Yang DT. An allozyme electrophoresis study on eleven species of megophryinae in China[J]. 动物学研究,1994,15(s1):182-192.
APA	Liu WZ,&Yang DT.(1994).An allozyme electrophoresis study on eleven species of megophryinae in China.动物学研究,15(s1),182-192.
MLA	Liu WZ,et al."An allozyme electrophoresis study on eleven species of megophryinae in China".动物学研究 15.s1(1994):182-192.