Role of Specific Synaptic Plasticity Interfering Peptides in the Expression of Morphine Induced Conditioned Place Preference in Mice

	Role of Specific Synaptic Plasticity Interfering Peptides in the Expression of Morphine Induced Conditioned Place Preference in Mice
	Wu K 1,2; Xu L 1; Huang JF[*]3
刊名	Zoological Research
	2009
卷号	30 期号:4 页码:389−395
关键词	LTP LTD Reward CPP
其他题名	特异突触可塑性干扰肽在小鼠吗啡条件化位置偏爱表达过程中的作用
通讯作者	huangjf@mail.kiz.ac.cn
合作状况	其它
中文摘要	在作为成瘾检测手段的条件化位置偏爱模型中,环境背景和成瘾药物间的关联性学习起着关键的作用.突触可塑性作为学习记忆可能的物质基础,在药物成瘾方面的研究也越来越多,但其表现形式,长时程增强(LTP)或者长时程抑制(LTD)在成瘾过程中所发挥的具体作用尚不得而知.因此,本文利用生物信息学手段,设计并合成了旨在分别阻断LTP和LTD的干扰肽,研究其对小鼠吗啡条件化位置偏爱的影响.结果发现,干扰肽Pep-A2和Pep-A3能够分别特异地阻断海马CA1区的LTP和LTD,在测试前尾静脉注射具有穿膜特性的LTP/LTD特异性干扰肽(Tat-A2/Tat-A3),均能阻断或损伤吗啡诱导的条件化位置偏爱的表达.此发现提示我们,LTP和LTD在成瘾性异常记忆的过程中均发挥着重要的作用.
英文摘要	Learned association between context and drug abuse is essential for the drug conditioned place preference (CPP), which is an animal model widely used to measure drug reward. Synaptic plasticity, in the form of long-term potentiation (LTP) and depression (LTD), is regarded as a proposed cellular substrate of learning and memory. However, the exact role of LTP/LTD in addiction is not known yet. Therefore, by bioinformatics we designed peptides aiming to interfere with LTP and LTD respectively, to study their individual role in the expression of morphine CPP. We found that the interfering peptide Pep-A2 can specifically block hippocampal LTP in CA1 region, whereas Pep-A3 can block LTD in this region. Treatment of either of their cell penetrating forms (Tat-A2 or Tat-A3) before test can block the expression of Morphine CPP in mice. These results suggested that both LTP and LTD are required in the drug-associated learning and memory.
收录类别	其他
资助信息	This work was supported by the National Natural Science Foundation of China(30530250; 30623007)
语种	英语
公开日期	2010-08-03
内容类型	期刊论文
源URL	[http://159.226.149.42:8088/handle/152453/829]
专题	昆明动物研究所_结构生物信息学昆明动物研究所_动物模型与人类重大疾病机理重点实验室昆明动物研究所_遗传资源与进化国家重点实验室昆明动物研究所_学习记忆的分子神经机制
作者单位	1.Key Laboratory of Animal Models and Human Disease Mechanisms, the Chinese Academy of Sciences & Yunnan Province, Kunming Institute of Zoology, Kunming 650223, China 2.Graduate School of the Chinese Academy of Sciences, Bejiing 100049, China 3.State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology , the Chinese Academy of Sciences, Kunming 650223, China
推荐引用方式 GB/T 7714	Wu K,Xu L,Huang JF[*]. Role of Specific Synaptic Plasticity Interfering Peptides in the Expression of Morphine Induced Conditioned Place Preference in Mice[J]. Zoological Research,2009,30(4):389−395.
APA	Wu K,Xu L,&Huang JF[*].(2009).Role of Specific Synaptic Plasticity Interfering Peptides in the Expression of Morphine Induced Conditioned Place Preference in Mice.Zoological Research,30(4),389−395.
MLA	Wu K,et al."Role of Specific Synaptic Plasticity Interfering Peptides in the Expression of Morphine Induced Conditioned Place Preference in Mice".Zoological Research 30.4(2009):389−395.