Nosema sp. PM-1, a new isolate of microsporidian from infected Papilio machaon Linnaeus, based on ultrastructure and molecular identification

	Nosema sp. PM-1, a new isolate of microsporidian from infected Papilio machaon Linnaeus, based on ultrastructure and molecular identification
	Liu T 1,2; Xu JS 3; Luo B 1; Chen J 1; Li Z 1; Li T 1; Pan GQ 1; Li XY 4; Zhou ZY[*]1,3
刊名	ACTA PARASITOLOGICA
	2015
卷号	60 期号:2 页码:330-336
关键词	Papilio machaon Linnaeus Phylogenetic analysis Nosema rRNA
通讯作者	zyzhou@swu.edu.cn
合作状况	其它
英文摘要	A new microsporidium, Nosema sp. PM-1, was first isolated from Papilio machaon Linnaeus. The spore shape of the PM-1 isolate was a long oval with an average size of 3.22 mu m x 1.96 mu m. Ultrastructure observation showed that PM-1 had a typical Nosema common diplokaryotic nuclei structure with 10-13 polar filament coils, spore wall, plasma membrane, and anchoring disk. The complete rRNA gene sequences were obtained by polymerase chain reaction amplification and each rRNA unit was arrayed as follows: 5'-LSU (2497 bp)-ITS (179 bp)-SSU (1232 bp)-IGS (278 bp)-5S (115 bp)-3', which was the same as typical Nosema. The phylogenetic trees of rRNA, DNA-directed RNA polymerase II subunit, and tubulin genes all show that PM-1 was a sister to the clade comprising Nosema bombycis, Nosema spodopterae, and Nosema sp. PX1. The spore morphology, ultrastructure, and complete rRNA structure indicate that this isolate assigned to the. true. Nosema group, can parasitized in Papilio machaon Linnaeus, which provides a wider host range for Nosema.
收录类别	SCI
资助信息	This work is supported by the grants from Na- tional Basic Research Program of China (No.2012CB114604), Nat- ural High-tech R&D Program (863 Program, No.2012AA101301 and No.2013AA102507), Natural Science Foundation of China (No. 312 72504, No. 30930067, No. 31001036, No. 31072089), the Pro- gram of IntroducingTalents of Discipline to Universities (No.B07045 ), Key Project of Ministry of Education of China (No. 210180), Science and Technology Project of Chongqing Municipal Education Commission (No.KJ100627 and KJ090813)
语种	英语
WOS记录号	WOS:000351855600021
公开日期	2015-04-28
内容类型	期刊论文
源URL	[http://159.226.149.42:8088/handle/152453/8315]
专题	昆明动物研究所_基因起源组
作者单位	1.State key laboratory of silkworm genome biology, Southwest University, Chongqing, 400716, China 2.School of mathematics and statistics, Southwest University, Chongqing, 400715, China 3.College of life sciences, Chongqing Normal University, Chongqing, 400047, China 4.Kunming Institute of Zoology, Yunnan, 650223, China
推荐引用方式 GB/T 7714	Liu T,Xu JS,Luo B,et al. Nosema sp. PM-1, a new isolate of microsporidian from infected Papilio machaon Linnaeus, based on ultrastructure and molecular identification[J]. ACTA PARASITOLOGICA,2015,60(2):330-336.
APA	Liu T.,Xu JS.,Luo B.,Chen J.,Li Z.,...&Zhou ZY[*].(2015).Nosema sp. PM-1, a new isolate of microsporidian from infected Papilio machaon Linnaeus, based on ultrastructure and molecular identification.ACTA PARASITOLOGICA,60(2),330-336.
MLA	Liu T,et al."Nosema sp. PM-1, a new isolate of microsporidian from infected Papilio machaon Linnaeus, based on ultrastructure and molecular identification".ACTA PARASITOLOGICA 60.2(2015):330-336.