Genetic variation and evolution of the Pit blast resistance locus in rice

	Genetic variation and evolution of the Pit blast resistance locus in rice
	Li JB 1; Sun YD 2; Liu H 3,4; Wang YY 2; Jia YL[]5; Xu MH[]2
刊名	GENETIC RESOURCES AND CROP EVOLUTION
	2014
卷号	61 期号:2 页码:473-489
关键词	Blast disease Evolution Magnaporthe oryzae Oryza sativa Pit Resistance gene
通讯作者	Yulin.Jia@ars.usda.gov ; xuminhui@sohu.com
合作状况	其它
英文摘要	Resistance (R) gene Pit in rice, encodes a protein with nucleotide binding sites-leucine rich repeat domain, prevents infections by strains of Magnaporthe oryzae in a gene for gene manner. Here, we analyzed the open reading frame (ORF) of Pit in 26 varieties including Oryza sativa L. subsp. indica Kato (Aus), indica (IND), temperate japonica (TEJ), tropical japonica (TRJ), aromatic (ARO) subgroups of Oryza sativa, and nine accessions of wild rice relatives including O. nivara and O. rufipogon from different growing areas. Twenty-one nucleotide differences were found within the ORFs of 35 accessions. Translation of these ORFs revealed a total of 11 Pit variants, seven of which were novel. Furthermore, variant H1 was identified in O. sativa and O. nivara; H2 and H10 were identified in O. sativa and O. rufipogon. H3, H4, and H11 were only found in O. sativa. H11 was the common type of TEJ and ARO; H3 and H4 was the specific type of TRJ and IND, respectively. H5, H6, and H7 were specific haplotypes of O. nivara, while, H8 and H9 were specific haplotypes of O. rufipogon. These results suggest that Pit is an ancient gene that exists before formation of rice domestication subgroups.
收录类别	SCI
资助信息	The authors gratefully thank Professor WangWenofKunmingInstituteofZoologyofChineseAcademy of Sciences for providing genome sequences of rice germplasm; Harold Bockelman of the USDA-ARS National Small Grains Collection, Aberdeen, Idaho, USA for providing germplasm accessions; Zaiquan Cheng of Institute of Biotechnology and Genetic Resources, YAAS; Yiqing Guo of Yunnan Agricultural Universityfor usefuldiscussions;and MichaelLin, Tracy Bianco and Ellen McWhirter of USDA-ARS Dale Bumpers National RiceResearchCenterfor pathogenicityassaysand proof reading, respectively. This work was supported by the National Natural Science Foundation of China (31160355) and Natural Science Fund of Yunnan of China (2010ZC173). USDA is an equal opportunity provider and employer.
语种	英语
WOS记录号	WOS:000330953400015
公开日期	2014-03-13
内容类型	期刊论文
源URL	[http://159.226.149.42:8088/handle/152453/7817]
专题	昆明动物研究所_基因起源组
作者单位	1.Agricultural Environment and Resources Research Institute, Yunnan Academy of Agricultural Sciences (YAAS), Kunming 650205, Yunnan Province, China 2.The Key Laboratory of Biotechnology Research of Yunnan Province, Key Lab of Southwestern Crop Gene Resources and Germplasm Innovation of Ministry of Agriculture, Institute of Biotechnology and Genetic Resources, Yunnan Academy of Agricultural Sciences (YAAS), Kunming 650223, Yunnan Province, China 3.Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, Yunnan Province, China 4.Graduate University of Chinese Academy of Sciences, Beijing 100049, China 5.United States Department of Agriculture–Agriculture Research Service, Dale Bumpers National Rice Research Center, Stuttgart, AR, USA
推荐引用方式 GB/T 7714	Li JB,Sun YD,Liu H,et al. Genetic variation and evolution of the Pit blast resistance locus in rice[J]. GENETIC RESOURCES AND CROP EVOLUTION,2014,61(2):473-489.
APA	Li JB,Sun YD,Liu H,Wang YY,Jia YL[],&Xu MH[].(2014).Genetic variation and evolution of the Pit blast resistance locus in rice.GENETIC RESOURCES AND CROP EVOLUTION,61(2),473-489.
MLA	Li JB,et al."Genetic variation and evolution of the Pit blast resistance locus in rice".GENETIC RESOURCES AND CROP EVOLUTION 61.2(2014):473-489.