De novo origination of a new protein-coding gene in Saccharomyces cerevisiae

	De novo origination of a new protein-coding gene in Saccharomyces cerevisiae
	Cai J 1,2; Zhao RP 1; Jiang HF 1,2; Wang W[*]1
刊名	GENETICS
	2008
卷号	179 期号:1 页码:487-496
ISSN号	0016-6731
通讯作者	wwang@mail.kiz.ac.cn
英文摘要	Origination of new genes is an important mechanism generating genetic novelties during the evolution of an organism. Processes of creating new genes using preexisting genes as the raw materials are well characterized, such as exon shuffling, gene duplication, retroposition, gene fusion, and fission. However, the process of how a new gene is de novo created from noncoding sequence is largely unknown. On the basis of genome comparison among yeast species, we have identified a new de noon protein-coding gene, BSC4 in Saccharomyces cerevisiae. The BSC4 gene has an open reading frame (ORF) encoding a 132-amino-acid-long peptide, while there is no homologous ORF in all the sequenced genomes of other fungal species, including its closely related species such as S. paradoxus and S. mikatae. The functional protein-coding feature of the BSC4 gene in S. cerevisiae is supported by population genetics, expression, proteomics, and synthetic lethal data. The evidence suggests that BSC4 may be involved in the DNA repair pathway during the stationary phase of S. cerevisiae and contribute to the robustness of S. cerevisiae, when shifted to a nutrient-poor environment. Because the corresponding noncoding sequences in S. paradoxus, S. mikatae, and S. boyanus also transcribe, we propose that a new de novo protein-coding gene may have evolved from a previously expressed noncoding sequence.
收录类别	SCI
资助信息	This work was supported by a Chinese Academy of Sciences–Max Planck Society Fellowship, by two National Natural Science Foundation of China key grants (nos. 30430400 and 30623007), and by a 973 Program (no. 2007CB815703-5) to W.W.
原文出处	2008179487.pdf
语种	英语
公开日期	2010-08-24
内容类型	期刊论文
源URL	[http://159.226.149.42:8088/handle/152453/4843]
专题	昆明动物研究所_基因起源组昆明动物研究所_遗传资源与进化国家重点实验室
作者单位	1.CAS–Max Planck Junior Research Group on Evolutionary Genomics, State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences (CAS), Kunming, Yunnan 650223, China 2.Graduate School of Chinese Academy of Sciences, Beijing 100049, China
推荐引用方式 GB/T 7714	Cai J,Zhao RP,Jiang HF,et al. De novo origination of a new protein-coding gene in Saccharomyces cerevisiae[J]. GENETICS,2008,179(1):487-496.
APA	Cai J,Zhao RP,Jiang HF,&Wang W[*].(2008).De novo origination of a new protein-coding gene in Saccharomyces cerevisiae.GENETICS,179(1),487-496.
MLA	Cai J,et al."De novo origination of a new protein-coding gene in Saccharomyces cerevisiae".GENETICS 179.1(2008):487-496.