Independency of anti-HIV-1 activity from ribosome-inactivating activity of trichosanthin

CORC > 昆明动物研究所 > 昆明动物研究所 > 动物模型与人类重大疾病机理重点实验室 > 分子免疫药理学

	Independency of anti-HIV-1 activity from ribosome-inactivating activity of trichosanthin
	Wang JH 1; Nie HL 2; Huang H 3; Tam SC 3; Zheng YT[*]1
刊名	BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
	2003
卷号	302 期号:1 页码:89-94
关键词	trichosanthin ribosome inactivating protein human immunodeficiency virus type 1 antiviral protein
通讯作者	zhengyt@mail.kiz.ac.cn
合作状况	其它
英文摘要	Trichosanthin (TCS) is a type I ribosome-inactivating (RI) protein possessing multiple biological and pharmacological activities. Its major action is inhibition of human immunodeficiency virus (HIV) replication but the mechanism is still elusive. All evidences showed that this action is related to its RI activity. Previous studies found that TCS mutants with reduced RI activity simultaneously lost some anti-HIV activity. In this study, an exception was demonstrated by two TCS mutants retaining almost all RI activity but were devoid of anti-HIV-1 activity. Five mutants were constructed by using site-directed mutagenesis with either deletion or addition of amino acids to the C-terminal sequence. Results showed that the RI activity of mutants with C-terminal deletion mutants (TCSC2, TCSC4, and TCSC14) decreased by 1.2-3.3-fold with parallel downshifting of its anti-HIV-1 activity (1.4-4.8-fold). Another two mutants, TCSC19aa and TCSKDEL having 19 amino acid extension and a KDEL signal sequence added to the C-terminal sequence, retained all RI activity but subsequently lost most of the anti-HIV-1 activity. These findings suggested that ribosome inactivation alone might not be adequate to explain the anti-HIV action of TCS.
收录类别	SCI
资助信息	This work was supported by grants from the National Natural Science Foundation of China (39970851), Natural Science Foundation of Yunnan Province (1999C0087M), and CAS Knowledge Innovation Projects (KSCX2- SW-216) to Dr. Y.T. Zheng.
语种	英语
内容类型	期刊论文
源URL	[http://159.226.149.26:8080/handle/152453/9676]
专题	昆明动物研究所_分子免疫药理学
作者单位	1.Laboratory of Molecular Immunopharmacology, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China 2.Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai 200031, China 3.Department of Physiology, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong, China
推荐引用方式 GB/T 7714	Wang JH,Nie HL,Huang H,et al. Independency of anti-HIV-1 activity from ribosome-inactivating activity of trichosanthin[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2003,302(1):89-94.
APA	Wang JH,Nie HL,Huang H,Tam SC,&Zheng YT[*].(2003).Independency of anti-HIV-1 activity from ribosome-inactivating activity of trichosanthin.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,302(1),89-94.
MLA	Wang JH,et al."Independency of anti-HIV-1 activity from ribosome-inactivating activity of trichosanthin".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 302.1(2003):89-94.