Cloning, sequencing, and polymorphism analysis of novel classical MHC class I alleles in northern pig-tailed macaques (Macaca leonina)

CORC > 昆明动物研究所 > 昆明动物研究所 > 动物模型与人类重大疾病机理重点实验室 > 分子免疫药理学

	Cloning, sequencing, and polymorphism analysis of novel classical MHC class I alleles in northern pig-tailed macaques (Macaca leonina)
	Lian XD 1,2; Zhang XH 1,2; Dai ZX 1; Zheng YT[*]1
刊名	IMMUNOGENETICS
	2016
卷号	68 期号:4 页码:261-274
关键词	AIDS model MHC class I Macaca leonina Northern pig-tailed macaque
通讯作者	zhengyt@mail.kiz.ac.cn
合作状况	其它
英文摘要	The northern pig-tailed macaque (Macaca leonina) has been confirmed to be an independent species from the pig-tailed macaque group of Old World monkey. We have previously reported that the northern pig-tailed macaques were also susceptible to HIV-1. Here, to make this animal a potential HIV/AIDS model and to discover the mechanism of virus control, we attempted to assess the role of major histocompatibility complex (MHC) class I-restricted immune responses to HIV-1 infection, which was associated with viral replication and disease progression. As an initial step, we first cloned and characterized the classical MHC class I gene of northern pig-tailed macaques. In this study, we identified 39 MHC class I alleles including 17 MHC-A and 22 MHC-B alleles. Out of these identified alleles, 30 were novel and 9 were identical to alleles previously reported from other macaque species. The MHC-A and MHC-B loci were both duplicates as rhesus macaques and southern pig-tailed macaques. In addition, we also detected the patterns of positive selection in northern pig-tailed macaques and revealed the existence of balance selection with 20 positive selection sites in the peptide binding region. The analysis of B and F peptide binding pockets in northern and southern pig-tailed macaques and rhesus macaques suggested that they were likely to share a few common peptides to present. Thus, this study provides important MHC immunogenetics information and adds values to northern pig-tailed macaques as a promising HIV/AIDS model.
收录类别	SCI
资助信息	This work was supported by the National Basic Research Program of China (2012CBA01305), the National Natural Science Foundation of China (81172876, 81373124, 81471620, 81571606), the Knowledge In- novation Program of CAS (KSCX2-EW-R-13, KJZD-EW-L10-02), the Natural Science Foundation of Yunnan Province (2014FB181), and the Collaborative Innovation Center for Natural Products and Biological Drugs of Yunnan.
语种	英语
内容类型	期刊论文
源URL	[http://159.226.149.26:8080/handle/152453/9532]
专题	昆明动物研究所_分子免疫药理学昆明动物研究所_动物模型与人类重大疾病机理重点实验室
作者单位	1.Chinese Acad Sci, Chinese Acad Sci & Yunnan Prov, Key Lab Anim Models & Human Dis Mech, Kunming Inst Zool, Kunming 650223, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Lian XD,Zhang XH,Dai ZX,et al. Cloning, sequencing, and polymorphism analysis of novel classical MHC class I alleles in northern pig-tailed macaques (Macaca leonina)[J]. IMMUNOGENETICS,2016,68(4):261-274.
APA	Lian XD,Zhang XH,Dai ZX,&Zheng YT[*].(2016).Cloning, sequencing, and polymorphism analysis of novel classical MHC class I alleles in northern pig-tailed macaques (Macaca leonina).IMMUNOGENETICS,68(4),261-274.
MLA	Lian XD,et al."Cloning, sequencing, and polymorphism analysis of novel classical MHC class I alleles in northern pig-tailed macaques (Macaca leonina)".IMMUNOGENETICS 68.4(2016):261-274.